Electrospray induced surface activation of polystyrene microbeads for diagnostic applications

dc.creatorFoti, Leonardo
dc.creatorSionek, Andre
dc.creatorStori, Elis Moura
dc.creatorSoares, Paula Poli
dc.creatorPereira, Miriam Marzall
dc.creatorKrieger, Marco Aurélio
dc.creatorPetzhold, Cesar Liberato
dc.creatorSchreiner, Wido Herwig
dc.creatorSoares, Maurilio José
dc.creatorGoldenberg, Samuel
dc.creatorSaul, Cyro Ketzer
dc.date2016-09-14T16:43:05Z
dc.date2016-09-14T16:43:05Z
dc.date2015
dc.date.accessioned2023-09-26T23:49:22Z
dc.date.available2023-09-26T23:49:22Z
dc.identifierFOTI, Leonardo et al. Electrospray induced surface activation of polystyrene microbeads for diagnostic applications. Journal of Materials Chemistry B, n. 3, p. 2725-2731, 2015.
dc.identifier2050-750X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15792
dc.identifier10.1039/c4tb01884b
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894592
dc.descriptionINCT para Diagnósticos em Saúde Pública (National Institute of Science and Technology for Diagnostics in Public Health), funded by the National Council of Technological and Scientific Development (CNPq – Brasil)
dc.descriptionElectrospray is generally regarded as a “soft” technique due to the absence of any observable molecular fragmentation or destruction. This study reports on a novel and easy way to induce surface activation on the surface of polystyrene microbeads through electrospray deposition into a grounded aqueous electrolyte solution bath. This process, nicknamed EISA, which stands for electrospray induced surface activation, proposes that when a highly charged microbead formed by the electrospray process sinks into the aqueous electrolyte solution, it behaves like a highly charged spherical capacitor that discharges in the conductive liquid. The energy released leads to a breakup of the polystyrene surface bonds and water oxidation with oxygen. Further reactions produce a carboxylated surface that was confirmed by X-ray photoelectron spectroscopy (XPS) and protein coupling. An immunoassay based on these modified microbeads was also developed and presented for use in syphilis detection, demonstrating a reliable signal-to-noise ratio between positive and negative results.
dc.formatapplication/pdf
dc.languagepor
dc.publisherChristine Schmidt
dc.rightsrestricted access
dc.subjectElectrospray
dc.subjectX-ray photoelectron spectroscopy
dc.subjectPolystyrene
dc.subjectSyphilis
dc.titleElectrospray induced surface activation of polystyrene microbeads for diagnostic applications
dc.typeArticle


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