dc.creatorFoti, Leonardo
dc.creatorSionek, Andre
dc.creatorStori, Elis Moura
dc.creatorSoares, Paula Poli
dc.creatorPereira, Miriam Marzall
dc.creatorKrieger, Marco Aurélio
dc.creatorPetzhold, Cesar Liberato
dc.creatorSchreiner, Wido Herwig
dc.creatorSoares, Maurilio José
dc.creatorGoldenberg, Samuel
dc.creatorSaul, Cyro Ketzer
dc.date2016-09-14T16:43:05Z
dc.date2016-09-14T16:43:05Z
dc.date2015
dc.date.accessioned2023-09-26T23:49:22Z
dc.date.available2023-09-26T23:49:22Z
dc.identifierFOTI, Leonardo et al. Electrospray induced surface activation of polystyrene microbeads for diagnostic applications. Journal of Materials Chemistry B, n. 3, p. 2725-2731, 2015.
dc.identifier2050-750X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15792
dc.identifier10.1039/c4tb01884b
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894592
dc.descriptionINCT para Diagnósticos em Saúde Pública (National Institute of Science and Technology for Diagnostics in Public Health), funded by the National Council of Technological and Scientific Development (CNPq – Brasil)
dc.descriptionElectrospray is generally regarded as a “soft” technique due to the absence of any observable molecular fragmentation or destruction. This study reports on a novel and easy way to induce surface activation on the surface of polystyrene microbeads through electrospray deposition into a grounded aqueous electrolyte solution bath. This process, nicknamed EISA, which stands for electrospray induced surface activation, proposes that when a highly charged microbead formed by the electrospray process sinks into the aqueous electrolyte solution, it behaves like a highly charged spherical capacitor that discharges in the conductive liquid. The energy released leads to a breakup of the polystyrene surface bonds and water oxidation with oxygen. Further reactions produce a carboxylated surface that was confirmed by X-ray photoelectron spectroscopy (XPS) and protein coupling. An immunoassay based on these modified microbeads was also developed and presented for use in syphilis detection, demonstrating a reliable signal-to-noise ratio between positive and negative results.
dc.formatapplication/pdf
dc.languagepor
dc.publisherChristine Schmidt
dc.rightsrestricted access
dc.subjectElectrospray
dc.subjectX-ray photoelectron spectroscopy
dc.subjectPolystyrene
dc.subjectSyphilis
dc.titleElectrospray induced surface activation of polystyrene microbeads for diagnostic applications
dc.typeArticle


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