Variation sites at the HLA-G 3' untranslated region confer differential susceptibility to HIV/HPV co-infection and aneuploidy in cervical cell

dc.creatorMedeiros, Fernanda Silva
dc.creatorMartins, Albert Eduardo Silva
dc.creatorGomes, Renan Garcia
dc.creatorOliveira, Sávio Augusto Vieira de
dc.creatorWelkovic, Stefan
dc.creatorMaruza, Magda
dc.creatorMenezes, Maria Luiza Bezerra
dc.creatorXimenes, Ricardo Arraes de Alencar
dc.creatorDiniz, George Tadeu Nunes
dc.creatorDonadi, Eduardo Antônio
dc.creatorLucena-Silva, Norma
dc.date2019-04-08T12:43:47Z
dc.date2019-04-08T12:43:47Z
dc.date2018
dc.date.accessioned2023-09-26T23:48:16Z
dc.date.available2023-09-26T23:48:16Z
dc.identifierMEDEIROS, F. S. et al. Variation Sites at the HLA-G 3’ Untranslated Region Confer Differential Susceptibility to HIV/HPV Co-Infection and Aneuploidy in Cervical Cell. PloS One, v. 13, n. 10, p. 1-14, 2018.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32404
dc.identifier10.1371/journal.pone.0204679
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894391
dc.descriptionPost-transcriptional regulatory elements associated with transcript degradation or transcript instability have been described at the 3' untranslated region (3'UTR) of the HLA-G gene. Considering that HPV infection and aneuploidy, which causes gene instability, are associated with cervical cell malignancy, as well as the fact that HIV infection and HLA-G may modulate the immune response, the present study aimed to compare the frequencies of HLA-G 3'UTR polymorphic sites (14-base pair insertion/deletion, +3142C/G, and +3187A/G) between 226 HIV+ women co-infected (n = 82) or not with HPV (n = 144) and 138 healthy women. We also evaluated the relationship between those HLA-G 3'UTR variants and aneuploidy in cervical cells. HPV types and HLA-G polymorphisms were determined by PCR and sequencing of cervical samples DNA. Aneuploidy in cervical cell was measured by flow cytometry. The HLA-G 3'UTR 14-bp ins/del was not associated with either HIV nor HIV/HPV co-infection. The +3142G allele (p = 0.049) and +3142GG genotype (p = 0.047) were overrepresented in all HIV-infected women. On the other hand, the +3187G allele (p = 0.028) and the +3187GG genotype (p = 0.026) predominated among healthy women. The +3142G (p = 0.023) and +3187A (p = 0.003) alleles were associated with predisposition to HIV infection, irrespective of the presence or not of HIV/HPV co-infection. The diplotype formed by the combination of the +3142CX (CC or CG) and +3187AA genotype conferred the highest risk for aneuploidy in cervical cell induced by HPV. The HLA-G 3'UTR +3142 and +3187 variants conferred distinct susceptibility to HIV infection and aneuploidy.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectHLA-G 3'
dc.subjectHIV/HPV
dc.subjectAneuploidy in cervical cell
dc.subject3 'Regiões não traduzidas
dc.subjectAdulto
dc.subjectAneuploidia
dc.subjectBrasil
dc.subjectEstudos de caso-controle
dc.subjectCervix Uteri / imunologia
dc.subjectUteri do colo do útero / virologia
dc.subjectCoinfecção / genética
dc.subjectCoinfecção / imunologia
dc.subjectFêmea
dc.subjectPredisposição Genética para Doença
dc.subjectInfecções por HIV / genética
dc.subjectInfecções por HIV / imunologia
dc.subjectAntígenos HLA-G / genética
dc.subjectHumanos
dc.subjectInfecções por papilomavírus / genética
dc.subjectInfecções por papilomavírus / imunologia
dc.subjectPolimorfismo Genético
dc.titleVariation sites at the HLA-G 3' untranslated region confer differential susceptibility to HIV/HPV co-infection and aneuploidy in cervical cell
dc.typeArticle


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