Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil

dc.creatorNogueira, Rita de Maria Seabra
dc.creatorSilva, Arannadia Barbosa
dc.creatorSato, Tayra Pereira
dc.creatorSá, Joicy Cortez de
dc.creatorSantos, Ana Clara Gomes dos
dc.creatorAmorim Filho, Edvaldo Franco
dc.creatorVale, Tássia Lopes do
dc.creatorGazeta, Gilberto Salles
dc.date2018-06-26T11:53:12Z
dc.date2018-06-26T11:53:12Z
dc.date2017
dc.date.accessioned2023-09-26T23:47:55Z
dc.date.available2023-09-26T23:47:55Z
dc.identifierNOGUEIRA, Rita de Maria Seabra; et al. Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. Pesquisa Veterinária Brasileira, v.37, n.12, p.1416-1422, 2017.
dc.identifier0100-736X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27094
dc.identifier10.1590/S0100-736X2017001200010
dc.identifier1678-5150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894330
dc.descriptionEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.
dc.formatapplication/pdf
dc.languageeng
dc.publisherColégio Brasileiro de Patologia Animal
dc.rightsopen access
dc.subjectCavalos
dc.subjectAnaplasma phagocytophilum
dc.subjectCarrapatos
dc.subjectBrasil
dc.subjectBabesia caballi
dc.subjectTheileria equi
dc.subjectDoença transmitida por vetor
dc.subjectVector-borne disease
dc.subjectTheileria equi
dc.subjectBabesia caballi
dc.subjectAnaplasma phagocytophilum
dc.subjecthorses
dc.subjectticks
dc.subjectBrazil
dc.titleMolecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil
dc.typeArticle


Este ítem pertenece a la siguiente institución