| dc.creator | Azevedo, Adriana S. | |
| dc.creator | Gonçalves, Antonio J. S. | |
| dc.creator | Archer, Marcia | |
| dc.creator | Freire, Marcos S. | |
| dc.creator | Galler, Ricardo | |
| dc.creator | Alves, Ada M. B. | |
| dc.date | 2015-09-21T17:25:30Z | |
| dc.date | 2015-09-21T17:25:30Z | |
| dc.date | 2013 | |
| dc.date.accessioned | 2023-09-26T23:47:20Z | |
| dc.date.available | 2023-09-26T23:47:20Z | |
| dc.identifier | AZEVEDO, Adriana S.; et al. The Synergistic Effect of Combined Immunization with a DNA Vaccine and Chimeric Yellow Fever/Dengue Virus Leads to Strong Protection against Dengue. PLoS ONE, v.8, n.3, e58357, 10p, march 2013. | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/11725 | |
| dc.identifier | 10.1371/journal.pone.0058357 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8894222 | |
| dc.description | The dengue envelope glycoprotein (E) is the major component of virion surface and its ectodomain is composed of
domains I, II and III. This protein is the main target for the development of a dengue vaccine with induction of neutralizing
antibodies. In the present work, we tested two different vaccination strategies, with combined immunizations in a prime/
booster regimen or simultaneous inoculation with a DNA vaccine (pE1D2) and a chimeric yellow fever/dengue 2 virus
(YF17D-D2). The pE1D2 DNA vaccine encodes the ectodomain of the envelope DENV2 protein fused to t-PA signal peptide,
while the YF17D-D2 was constructed by replacing the prM and E genes from the 17D yellow fever vaccine virus by those
from DENV2. Balb/c mice were inoculated with these two vaccines by different prime/booster or simultaneous
immunization protocols and most of them induced a synergistic effect on the elicited immune response, mainly in
neutralizing antibody production. Furthermore, combined immunization remarkably increased protection against a lethal
dose of DENV2, when compared to each vaccine administered alone. Results also revealed that immunization with the DNA
vaccine, regardless of the combination with the chimeric virus, induced a robust cell immune response, with production of
IFN-c by CD8+ T lymphocytes. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Plos One | |
| dc.rights | open access | |
| dc.subject | Dengue | |
| dc.subject | DNA Vaccine | |
| dc.subject | Chimeric Yellow Fever/Dengue Virus | |
| dc.subject | Febre Amarela | |
| dc.subject | Vírus da Dengue | |
| dc.subject | Vacinas | |
| dc.title | The Synergistic Effect of Combined Immunization with a DNA Vaccine and Chimeric Yellow Fever/Dengue Virus Leads to Strong Protection against Dengue | |
| dc.type | Article | |
