Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups

dc.creatorFampa, P.
dc.creatorLisboa, C. V.
dc.creatorJansen, Ana Maria
dc.creatorSantos, A. L. S.
dc.creatorRamirez, M. I.
dc.date2019-03-14T13:40:52Z
dc.date2019-03-14T13:40:52Z
dc.date2008
dc.date.accessioned2023-09-26T23:46:48Z
dc.date.available2023-09-26T23:46:48Z
dc.identifierFAMPA, P. et al. Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups. Parasitology, v. 135, p. 1093–1100, 2008.
dc.identifier0031-1820
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32080
dc.identifier10.1017/S0031182008004587
dc.identifier1469-8161
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894124
dc.descriptionProtease expression among TCI and TCII field isolates was analysed. Gelatin-containing gels revealed hydrolysis bands with molecular masses ranging from 45 to 66 kDa. The general protease expression profile showed that TCII isolates presented higher heterogeneity compared to TCI. By utilizing protease inhibitors, we showed that all active proteases at acid pH are cysteine-proteases and all proteases active at alkaline pH are metalloproteases. However, the expression of cruzipain, the T. cruzi major cysteine-protease, did not reproduce a heterogeneous TCII cysteine zymogram profile. Dendogram analyses based on presence/absence matrices of proteases and cruzipain bands showed a TCI separation from the TCII group with 50–60% similarity. We suggest that the observed cysteine protease diversification contributes to differential host infection between TCI and II genotypes.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectInteração parasita-hospedeiro
dc.subjectFatores de Virulência
dc.subjectProteases
dc.subjectGenótipos TCI e TCII
dc.subjectTrypanosoma cruzi
dc.subjectTCI and TCII genotypes
dc.subjectProteases
dc.subjectCruzipain
dc.subjectHost-parasite interaction
dc.subjectVirulence
dc.titleProtease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups
dc.typeArticle


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