Patterns of expression of odorant receptor genes in a Chagas disease vector.

dc.creatorEstivalis, José Manuel Latorre
dc.creatorOliveira, Emerson Soares de
dc.creatorEsteves, Barbara Beiral
dc.creatorGuimarães, Letícia Santos
dc.creatorRamos, Marina Neves
dc.creatorLorenzo, Marcelo Gustavo
dc.date2016-02-04T18:23:21Z
dc.date2016-02-04T18:23:21Z
dc.date2015
dc.date.accessioned2023-09-26T23:45:25Z
dc.date.available2023-09-26T23:45:25Z
dc.identifierESTIVALIS, Jose Manuel Latorre et al. Patterns of expression of odorant receptor genes in a Chagas disease vector. Insect Biochemistry and Molecular Biology, 2015.
dc.identifier0965-1748
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12705
dc.identifier10.1016/j.ibmb.2015.05.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893885
dc.descriptionRhodnius prolixus is a triatomine bug acting as a relevant vector of Chagas disease for which the genome sequence has been recently made available. Based on this information, a set of olfactory (ORs) and ionotropic receptor (IRs) genes potentially related to olfactory processes was characterized, and the expression patterns along bug development and in different structures potentially involved in promoting chemosensory-mediated behaviors were studied. For this, diverse bioinformatic procedures were used to validate gene models analyzing their structural and functional features and designing specific primers. Evolutionary relationships among R. prolixus olfactory coreceptors (RproOrco, RproIR25a, RproIR8a and RproIR76b) and their orthologues from other insects were shown to have mostly good bootstrap support values in phylogenetic trees. Moreover, antennal expression was confirmed for most genes included in the study. Both ORs and IRs showed antennal expression along the whole development of bugs of this species, with few exceptional receptors showing gradually increasing expression or expression restricted to the antennae of adult bugs. Finally, the expression of most of the selected genes was confirmed in other structures, such as rostri, tarsi, tibial pads and genitalia, which are potentially involved in promoting chemosensory-mediated behaviors. These results are discussed in terms of their relevance to advance in the understanding of the molecular bases of triatomine behavior.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Ltd.
dc.rightsrestricted access
dc.subjectOlfaction
dc.subjectReceptors
dc.subjectTriatomines
dc.subjectPhysiology and Gene Expression
dc.titlePatterns of expression of odorant receptor genes in a Chagas disease vector.
dc.typeArticle


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