r-Sm14 - pRSETA Efficacy in Experimental Animals

dc.creatorRamos, Celso Raul Romero
dc.creatorVilar, Mônica Magno
dc.creatorNascimento, Ana Lúcia Tabet Oller
dc.creatorHo, Paulo Lee
dc.creatorThaumaturgo, Nilton
dc.creatorEdelenyi, Ricardo
dc.creatorAlmeida, Marília
dc.creatorDias, Waldely de Oliveira
dc.creatorDiogo, Catia Maria
dc.creatorTendler, Miriam
dc.date2020-10-24T18:11:23Z
dc.date2020-10-24T18:11:23Z
dc.date2001
dc.date.accessioned2023-09-26T23:45:02Z
dc.date.available2023-09-26T23:45:02Z
dc.identifierRAMOS, Celso Raul Romero et al. r-Sm14 - pRSETA Efficacy in Experimental Animals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, Suppl., p. 131-135, 2001.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44170
dc.identifier10.1590/S0074-02762001000900019
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893820
dc.descriptionFasciola hepatica infections were performed with recombinant Sm14 (rSm14) produced in Escherichia coli by the pGEMEX system (Promega). The rSm14 was expressed as a 40 kDa fusion protein with the major bacteriophage T7 capsid protein. Vaccination experiments with this rSm14 in animal models resulted in consistent high protective activity against S. mansoni cercariae challenge and enabled rSm14 to be included among the vaccine antigens endorsed by the World Health Organization for phase I/II clinical trials. Since the preparation of pGEMEX based rSm14 is time consuming and results in low yield for large scale production, we have tested other E. coli expression systems which would be more suitable for scale up and downstream processing. We expressed two different 6XHis-tagged Sm14 fusion proteins in a T7 promoter based plasmids. The 6XHis-tag fusions allowed rapid purification of the recombinant proteins through a Ni+2-charged resin. The resulted recombinant 18 and 16 kDa proteins were recognized by anti-Sm14 antibodies and also by antiserum against adult S. mansoni soluble secreted/excreted proteins in Western-Blot. Both proteins were also protective against S. mansoni cercariae infection to the same extent as the rSm14 expressed by the pGEMEX system.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectSchistosoma mansoni
dc.subjectProteína de ligação de ácido graxo
dc.subjectVacina anti-helminto
dc.subjectSchistosoma mansoni
dc.subjectFatty acid binding protein
dc.subjectAnti-helminth vaccine
dc.titler-Sm14 - pRSETA Efficacy in Experimental Animals
dc.typeArticle


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