SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics

dc.creatorGreenwood, J.
dc.creatorPryce, G.
dc.creatorDevine, L.
dc.creatorMale, D. K.
dc.creatorSantos, Washington Luis Conrado dos
dc.creatorCalder, V. L.
dc.creatorAdamson, P.
dc.date2017-07-11T13:03:00Z
dc.date2017-07-11T13:03:00Z
dc.date1996
dc.date.accessioned2023-09-26T23:43:42Z
dc.date.available2023-09-26T23:43:42Z
dc.identifierGREENWOOD, J. et al. SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology, v. 71, p. 51-63, 1996.
dc.identifier0165-5728
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20037
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893586
dc.descriptiondosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
dc.descriptionIn the central nervous system the blood–brain and blood–retinal barriers (BBBand BRBrespectively)are instrumentalin maintaining homeostasisof the neural parenchymaand controllingleucocytetraffic. These cellular barriers are formed primarilyby the vascular endotheliumof the brain and retina althoughin the latter the pigmentedepithelial cells also form part of the barrier. From primary culturesof rat brain endothelium,retinal endotheliumand retinal pigmentepitheiium(RPE) we have generatedtemperaturesensitive SV40largeT immortalisedcell lines. Clonesof brain (GP8.3)and retinal(JG2.1)endotheliaand RPE (LD7.4)have been derivedfrom parent lines that expressthe large T antigen at the permissivetemperature.The endothelialcell (EC) lines expressedP-glycoprotein, GLUT-1,the transfernnreceptor,von Willebrandfactor and the RECA-1antigenand exhibitedhigh affinityuptakeof acetylatedLDL and stainedpositivewith the lectin Griffoniasimplicifolia.The RPE cell line was positivefor cytokeratinsand for the rat RPE antigen RET-PE2.All the cell lines expressedmajor histocompatibilitycomplex(MHC)class 1 and intercellularadhesionmolecule(ICAM)-1 constitutivelyand could be induced to expressMHC class II and vascular cell adhesion molecule (VCAM)-1following cytokine activation.The EC also expressedplatelet endothelialcelI adhesionmolecule(PECAM)-I.Monolayer of these cells could supportthe migrationof antigen-specificT cell lines.Thegenerationof immortalisedcell linesderivedfromthe rat BBBandBRBshouldproveto be usefultools for the studyof these specialisedcellularbarriers.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectBarreira hematoencefalica
dc.subjectBarreira sangue-retina
dc.subjectEndotélio
dc.subjectEpitélio pigmentado da retina
dc.subjectBlood–brain barrier
dc.subjectBlood–retinal barrier
dc.subjectEndothelium
dc.subjectImmortalisation
dc.subjectRetinal pigment epitheliums
dc.titleSV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
dc.typeArticle


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