Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs

dc.creatorSampaio, Julia Ramos
dc.creatorSoares, Rodrigo Pedro Pinto
dc.creatorBarral, Thiago Doria
dc.creatorPassos, Gabriela Porfirio
dc.creatorFonseca, Maisa Santos
dc.creatorMeyer, Roberto
dc.creatorBarrouin-Melo, Stella Maria
dc.creatorPortela, Ricardo Wagner
dc.date2022-02-11T13:22:13Z
dc.date2022-02-11T13:22:13Z
dc.date2021
dc.date.accessioned2023-09-26T23:41:42Z
dc.date.available2023-09-26T23:41:42Z
dc.identifierSAMPAIO, Julia Ramos et al. Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs. Front Vet Sci, v. 8, 581148, 2021. doi: 10.3389/fvets.2021.581148. eCollection 2021.
dc.identifier2297-1769
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51153
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893255
dc.descriptionLipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.
dc.formatapplication/pdf
dc.languagepor
dc.publisherFrontiers Media S.A
dc.rightsopen access
dc.subjectELISA
dc.subjectLeishmania infantum
dc.subjectdogs
dc.subjectglycoconjugates
dc.subjectimmunodiagnosis
dc.titleLeishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs
dc.typeArticle


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