1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

dc.creatorEl-Bacha, Tatiana
dc.creatorStruchiner, Claudio J.
dc.creatorCordeiro, Marli Tenorio
dc.creatorAlmeida, Fabio C. L.
dc.creatorMarques, Ernesto Torres
dc.creatorDa Poian, Andrea T.
dc.date2017-06-14T14:18:51Z
dc.date2017-06-14T14:18:51Z
dc.date2016
dc.date.accessioned2023-09-26T23:41:31Z
dc.date.available2023-09-26T23:41:31Z
dc.identifierEL-BACHA, Tatiana et al. 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients. Journal of Virology, v. 90, n. 16, p. 7429-7443, Aug. 2016.
dc.identifier1098-5514
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/19389
dc.identifier10.1128/JVI.00187-16
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893225
dc.descriptionDengue, due to its global burden, is the most important arthropod-borne flavivirus disease, and early detection lowers fatality rates to below 1%. Since the metabolic resources crucial for viral replication are provided by host cells, detection of changes in the metabolic profile associated with disease pathogenesis could help with the identification of markers of prognostic and diagnostic importance. We applied (1)H nuclear magnetic resonance exploratory metabolomics to study longitudinal changes in plasma metabolites in a cohort in Recife, Brazil. To gain statistical power, we used innovative paired multivariate analyses to discriminate individuals with primary and secondary infection presenting as dengue fever (DF; mild) and dengue hemorrhagic fever (DHF; severe) and subjects with a nonspecific nondengue (ND) illness (ND subjects). Our results showed that a decrease in plasma low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) discriminated dengue virus (DENV)-infected subjects from ND subjects, and also, subjects with severe infection even presented a decrease in lipoprotein concentrations compared to the concentrations in subjects with mild infection. These results add to the ongoing discussion that the manipulation of lipid metabolism is crucial for DENV replication and infection. In addition, a decrease in plasma glutamine content was characteristic of DENV infection and disease severity, and an increase in plasma acetate levels discriminated subjects with DF and DHF from ND subjects. Several other metabolites shown to be altered in DENV infection and the implications of these alterations are discussed. We hypothesize that these changes in the plasma metabolome are suggestive of liver dysfunction, could provide insights into the underlying molecular mechanisms of dengue virus pathogenesis, and could help to discriminate individuals at risk of the development of severe infection and predict disease outcome.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectDengue
dc.subjectMortalidade
dc.subjectDiagnóstico precoce
dc.subjectDengue / complicações
dc.subjectDengue / patologia
dc.subjectHepatopatias / diagnóstico
dc.subjectEspectroscopia de Ressonância Magnética
dc.subjectMetabolômica
dc.subjectPlasma / Química
dc.title1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
dc.typeArticle


Este ítem pertenece a la siguiente institución