A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test

dc.creatorFerrão Filho, Aloysio da S.
dc.creatorSoares, Maria Carolina S.
dc.creatorMagalhães, Valéria Freitas de
dc.creatorAzevedo, Sandra M. F. O.
dc.date2017-03-16T11:48:32Z
dc.date2017-03-16T11:48:32Z
dc.date2010
dc.date.accessioned2023-09-26T23:41:05Z
dc.date.available2023-09-26T23:41:05Z
dc.identifierFERRÃO FILHO, Aloysio da S. et al. A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test. Environmental Pollution, v.158, n.6, p.2084-2093, 2010.
dc.identifier0269-7491
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18053
dc.identifier10.1016/j.envpol.2010.03.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8893146
dc.descriptionBioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET50) was adopted as the endpoint. Paralysis of swimming movements was observed between w0.5e3 h of exposure to lake water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity within 24 h after being placed in control water. The same effects were observed in bioassays with a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression analysis showed significant relationships between ET50 vs. cell density, biomass and saxitoxins content, suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins in natural samples, with important advantages over mouse bioassays.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectCianobactérias
dc.subjectDaphnia
dc.subjectCylindrospermopsis
dc.subjectCladóceros
dc.subjectSaxitoxinas
dc.subjectCyanobacteria
dc.subjectCladocerans
dc.subjectDaphnia
dc.subjectSaxitoxins
dc.subjectCylindrospermopsis
dc.titleA rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test
dc.typeArticle


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