Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis

dc.creatorFerreira, Gabriella Freitas
dc.creatorMoraes, Cícero
dc.creatorSilveira, Alda Maria Soares da
dc.creatorOliveira, Rodrigo Corrêa de
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorMartins Filho, Olindo Assis
dc.creatorMoreno, Elizabeth Castro
dc.creatorCarmo, Luiz Simeão do
dc.creatorFraga, Lucia Alves de Oliveira
dc.creatorMalaquias, Luiz Cosme Cotta
dc.date2014-03-21T12:42:24Z
dc.date2014-03-21T12:42:24Z
dc.date2012
dc.date.accessioned2023-09-26T23:40:12Z
dc.date.available2023-09-26T23:40:12Z
dc.identifierFERREIRA, Freitas Ferreira et al. Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.3, pp. 348-355.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7432
dc.identifier10.1590/S0074-02762012000300009
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892989
dc.descriptionWe investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and IL-10 secretion in both OST and non-infected individuals (NI). Even though stimulation with SEA had no impact on IL-6 production in either patient group, the baseline level of IL-6 production by cells from OST patients was always significantly less than that produced by cells from NI. After classifying the osteomyelitic episodes based on the time after the last reactivation event as "early" (1-4 months) or "late" osteomyelitis (5-12 months), we found that increased levels of TNF-α and IL-4 in combination with decreased levels of IL-6 were observed in the early episodes. By contrast, increased levels of IL-10, IL-2 and IL-6 were hallmarks of late episodes. Our data demonstrate that early osteomyelitic episodes are accompanied by an increased frequency of "high producers" of TNF-α and IL-4, whereas late events are characterised by increased frequencies of "high producers" of IL-10, IL-6 and IL-2. These findings demonstrate the distinct cytokine profiles in chronic osteomyelitis, with a distinct regulation of IL-6 production during early and late episodes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectStaphylococcus aureus
dc.subjectenterotoxin A
dc.subjectchronic osteomyelitis
dc.subjectcytokines
dc.subjectIL-6
dc.titleDistinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
dc.typeArticle


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