Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review

dc.creatorDegrave, Wim M.
dc.creatorFernandes, Octavio
dc.creatorCampbell, David
dc.creatorBozza, Marcelo
dc.creatorLopes, Ulisses
dc.date2019-11-03T15:00:25Z
dc.date2019-11-03T15:00:25Z
dc.date1994
dc.date.accessioned2023-09-26T23:39:57Z
dc.date.available2023-09-26T23:39:57Z
dc.identifierDEGRAVE, Wim et al. Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 3, p. 463-469, July/Sept. 1994.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36819
dc.identifier10.1590/S0074-02761994000300032
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892946
dc.descriptionThe use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectDNA de Cinetoplasto
dc.subjectLeishmania
dc.subjectReação em Cadeia da Polimerase
dc.subjectHibridização
dc.subjectSondas Moleculares
dc.subjectDNA de Cinetoplasto
dc.subjectLeishmania
dc.subjectPolymerase chain reaction
dc.subjectHybridization
dc.subjectMolecular probes
dc.subjectMini-exon gene
dc.subjectMinicircle
dc.subjectkDNA
dc.titleUse of molecular probes and PCR for detection and typing of Leishmania - a mini-review
dc.typeArticle


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