Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells

dc.creatorCarvalho, Renata Faria de
dc.creatorQuintaes, Lucas de Siqueira Penna
dc.creatorSu, Thaís de Cássia de Souza
dc.creatorKobayashi, Leticia Mitiko
dc.creatorNogueira, Ana Cristina Martins de Almeida
dc.date2022-11-09T17:59:12Z
dc.date2022-11-09T17:59:12Z
dc.date2022
dc.date.accessioned2023-09-26T23:39:32Z
dc.date.available2023-09-26T23:39:32Z
dc.identifierCARVALHO, Renata faria de et al. Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. Plos One, v. 17, n. 5, e0267653, p. 1 - 14, May 2022.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55558
dc.identifier10.1371/ journal.pone.0267653
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892872
dc.descriptionDengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial dengue vaccine and test the feasibility of this approach in the development of a potency assay with human cell lines, as a methodological alternative to the golden standard potency assay with VERO cells. In this context, we used a batch of the commercial vaccine Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined by immunocolorimetric assay using monoclonal antibodies specific for each serotype. The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine, as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to the one observed with VERO cells. Moreover, it was possible to determine the infectivity and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing over the others. Thus, the human cell line HEK293T provides a potential candidate to be used in assays to determine potency and identity of tetravalent dengue vaccines.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectInfectar diferentes linhagens celulares humanas
dc.subjectTeste de potência alternativo
dc.subjectUso de células HEK293T
dc.subjectViabilidade da vacina contra dengue
dc.subjectFeasibility of dengue vaccine
dc.subjectInfect different human cell lines
dc.subjectAlternative potency test
dc.subjectUsing HEK293T cells
dc.titleShort communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells
dc.typeArticle


Este ítem pertenece a la siguiente institución