The role of the Trypanosoma Cruzi TcNRBD1 Protein in translation

dc.creatorOliveira, Camila
dc.creatorCarvalho, Paulo Costa
dc.creatorAlves, Lysangela Ronalte
dc.creatorGoldenberg, Samuel
dc.date2017-07-24T18:10:26Z
dc.date2017-07-24T18:10:26Z
dc.date2016
dc.date.accessioned2023-09-26T23:39:03Z
dc.date.available2023-09-26T23:39:03Z
dc.identifierOLIVEIRA C. et al. The Role of the Trypanosoma cruzi TcNRBD1 Protein in Translation. PLoS ONE, v. 11, n.10, p. 1-12, Oct. 2016.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20381
dc.identifier10.1371/journal.pone.0164650
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892788
dc.descriptionThe regulation of gene expression in trypanosomatids occurs mainly at the post-transcriptional level. Despite the importance of this type of control in Trypanosoma cruzi, few RNA binding proteins have been characterized. The RRM domain (RNA Recognition Motif) is one of the most abundant domains found in RNA-binding proteins in higher eukaryotes. Proteins containing the RRM domain are involved in the majority of post-transcriptional processes regulating gene expression. In this work, we aimed to characterize the protein TcNRBD1 from T. cruzi. TcNRBD1 is an RNA-binding protein that contains 2 RRM domains and is the ortholog of the P34 and P37 proteins from Trypanosoma brucei. The TcNRBD1 protein is expressed in all developmental stages of T. cruzi, and its localization pattern is concentrated at the perinuclear region. TcNRBD1 is associated with polysomes and with the 80S monosomes. Furthermore, sequencing of the mRNAs bound to TcNRBD1 allowed the identification of several transcripts that encode ribosomal proteins. Immunoprecipitation assays followed by mass spectrometry showed that the protein complexes with several ribosomal proteins from both the 40S and 60S subunits. In summary, the results indicate that TcNRBD1 is associated with different parts of the translation process, either by regulating mRNAs that encode ribosomal proteins or by acting in some step of ribosome assembly in T. cruzi.
dc.formatapplication/pdf
dc.languagepor
dc.publisherUniversity of Toronto
dc.rightsopen access
dc.subjectRegulação da Expressão Gênica
dc.subjectGene Expression Regulation
dc.subjectRNA Recognition Motif Proteins
dc.subjectRibosomal Proteins
dc.subjectTrypanosoma cruzi
dc.subjectProteínas com Motivo de Reconhecimento de RNA
dc.subjectProteínas Ribossômicas
dc.titleThe role of the Trypanosoma Cruzi TcNRBD1 Protein in translation
dc.typeArticle


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