Proteogenomic analysis reveals proteins involved in the first step of adipogenesis in human adipose-derived stem cells

dc.creatorBonilauri, Bernardo
dc.creatorCamillo-Andrade, Amanda C.
dc.creatorSantos, Marlon Dias Mariano
dc.creatorFischer, Juliana de Saldanha da Gama
dc.creatorCarvalho, Paulo Costa
dc.creatorDallagiovanna, Bruno
dc.date2022-01-18T15:52:03Z
dc.date2022-01-18T15:52:03Z
dc.date2021
dc.date.accessioned2023-09-26T23:37:24Z
dc.date.available2023-09-26T23:37:24Z
dc.identifierBONILAURI, Bernardo et al. Proteogenomic analysis reveals proteins involved in the first step of adipogenesis in human adipose-derived Stem Cells. Stem Cells International, p. 1–14, 2021.
dc.identifier1687-9678
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50799
dc.identifier10.1155/2021/3168428
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892504
dc.descriptionObesity is characterized as a disease that directly affects the whole-body metabolism and is associated with excess fat mass and several related comorbidities. Dynamics of adipocyte hypertrophy and hyperplasia play an important role in health and disease, especially in obesity. Human adipose-derived stem cells (hASC) represent an important source for understanding the entire adipogenic differentiation process. However, little is known about the triggering step of adipogenesis in hASC. Here, we performed a proteogenomic approach for understanding the protein abundance alterations during the initiation of the adipogenic differentiation process. Methods. hASC were isolated from adipose tissue of three donors and were then characterized and expanded. Cells were cultured for 24 hours in adipogenic differentiation medium followed by protein extraction. We used shotgun proteomics to compare the proteomic profile of 24 h-adipogenic, differentiated, and undifferentiated hASC. We also used our previous next-generation sequencing data (RNA-seq) of the total and polysomal mRNA fractions of hASC to study posttranscriptional regulation during the initial steps of adipogenesis. We identified 3420 proteins out of 48,336 peptides, of which 92 proteins were exclusively identified in undifferentiated hASC and 53 proteins were exclusively found in 24 h-differentiated cells. Using a stringent criterion, we identified 33 differentially abundant proteins when comparing 24 h-differentiated and undifferentiated hASC (14 upregulated and 19 downregulated, respectively). Among the upregulated proteins, we shortlisted several adipogenesis-related proteins. A combined analysis of the proteome and the transcriptome allowed the identification of positive correlation coefficients between proteins and mRNAs. These results demonstrate a specific proteome profile related to adipogenesis at the beginning (24 hours) of the differentiation process in hASC, which advances the understanding of human adipogenesis and obesity. Adipogenic differentiation is finely regulated at the transcriptional, posttranscriptional, and posttranslational levels.
dc.formatapplication/pdf
dc.languagepor
dc.publisherHindawi
dc.rightsopen access
dc.subjectMesenchymal Stem Cells
dc.subjectProteogenomics
dc.subjectAdipogenesis
dc.subjectObesity
dc.subjectCélulas Madre Mesenquimatosas
dc.subjectProteogenómica
dc.subjectAdipogénesis
dc.subjectObesidad
dc.subjectCellules souches mésenchymateuses
dc.subjectProtéogénomique
dc.subjectAdipogenèse
dc.subjectObésité
dc.subjectCélulas-Tronco Mesenquimais
dc.subjectProteogenômica
dc.subjectAdipogênese
dc.subjectObesidade
dc.titleProteogenomic analysis reveals proteins involved in the first step of adipogenesis in human adipose-derived stem cells
dc.typeArticle


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