Impaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect

dc.creatorAlmeida, Vinícius Cotta de
dc.creatorVilla-Verde, Déa Maria Serra
dc.creatorLepault, Françoise
dc.creatorPléau, Jean-Marie
dc.creatorDardenne, Mireille
dc.creatorSavino, Wilson
dc.date2019-07-09T11:25:54Z
dc.date2019-07-09T11:25:54Z
dc.date2004
dc.date.accessioned2023-09-26T23:36:19Z
dc.date.available2023-09-26T23:36:19Z
dc.identifierALMEIDA, Viniicius Cotta de et al. Impaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect. Eur. J. Immunol., v. 34, p. 1578-1587, 2004.
dc.identifier0014-2980
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33898
dc.identifier10.1002/eji.200324765
dc.identifier1521-4141
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8892315
dc.descriptionWe previously showed intrathymic alterations in non-obese diabetic (NOD) mice, including the appearance of giant perivascular spaces, filled with mature thymocytes, intermingled with an extracellular matrix network. This raised the hypothesis of a defect in thymocyte migration with partial arrest of exiting thymocytes in the perivascular spaces. Herein, we investigated the expression of receptors for fibronectin [very late antigen (VLA)-4 and VLA-5] and laminin (VLA-6), known to play a role in thymocyte migration. When compared with two normal and one other autoimmune mouse strains, a decrease of VLA-5 expression in NOD thymocytes was noticed, being firstly observed in late CD4/CD8 double-negative cells, and more pronounced in mature CD4(+) and CD8(+) thymocytes. Functionally, thymocyte exit from the lymphoepithelial complexes, the thymic nurse cells, was reduced. Moreover, NOD thymocyte adhesion to thymic epithelial cells as well as to fibronectin was diminished, and so was the migration of NOD thymocytes through fibronectin-containing transwell chambers. In situ, intra-perivascular space thymocytes were VLA-5-negative, suggesting a correlation between the thymocyte arrest within these structures and loss of VLA-5 expression. Overall, our data reveal impairment in NOD thymocyte migration, and correspond to the first demonstration of a functional fibronectin receptor defect in the immune system.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley 12 Months
dc.rightsrestricted access
dc.subjectMigração de timócitos
dc.subjectFibronectina
dc.subjectIntegrinas
dc.subjectNOD mice
dc.subjectThymocyte migration
dc.subjectVLA
dc.subjectIntegrins
dc.subjectFibronectin
dc.subjectReceptores de Antígeno muito Tardio
dc.subjectCamundongos Endogâmicos NOD
dc.titleImpaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect
dc.typeArticle


Este ítem pertenece a la siguiente institución