dc.creator | Figueiredo, Fabiano Borges | |
dc.creator | Vasconcelos, Tassia Cristina Bello de | |
dc.creator | Madeira, Maria de Fátima | |
dc.creator | Menezes, Rodrigo Caldas | |
dc.creator | Maia-Elkhoury, Ana Nilce Silveira | |
dc.creator | Marcelino, Andreza Pain | |
dc.creator | Werneck, Guilherme L. | |
dc.date | 2019-06-28T19:44:44Z | |
dc.date | 2019-06-28T19:44:44Z | |
dc.date | 2018 | |
dc.date.accessioned | 2023-09-26T23:34:13Z | |
dc.date.available | 2023-09-26T23:34:13Z | |
dc.identifier | FIGUEIREDO, Fabiano Borges et al. Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 11, p. 1-7, 2018. | |
dc.identifier | 0074-0276 | |
dc.identifier | https://www.arca.fiocruz.br/handle/icict/33735 | |
dc.identifier | 10.1590/0074-02760180260 | |
dc.identifier | 1678-8060 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8891987 | |
dc.description | Visceral leishmaniasis is a major public health challenge in South America, and dogs are its main urban reservoir. It has the objective to do the validation of the canine Dual-path Platform immunoassay for canine visceral leishmaniasis (DPP® CVL) for a sample set composed of 1446 dogs from different Brazilian endemic areas. It had as methods a well-defined reference standard by means of parasitological culture, immunohistochemistry, and histopathology was used. Animals were classified as asymptomatic, oligosymptomatic, or symptomatic. Sensitivity and specificity were assessed as a single set and in clinical groups. A reproducibility assessment of the tests was conducted using the Kappa (κ) index at three different laboratories (A, B, and C). It was findings that overall, 89% sensitivity and 70% specificity were obtained for the entire sample set. Analysis of the clinical groups showed a gradual decrease in the sensitivity and an increase in the specificity with the reduction of clinical signs in the dogs that were assessed, reaching a sensitivity of 75% (42.8-94.5%) among asymptomatic dogs and lower specificity of 56% (46.2-66.3%) among symptomatic dogs. Inter-laboratory agreement was substantial (κAB= 0.778; κAC= 0.645; κCB= 0.711). The main conclusion was that the test performance is somewhat dependent on canine symptomatology, but such influence was less evident than in previous studies. Favourable results for sensitivity and specificity can be obtained even in asymptomatic animals; however, caution is needed in these evaluations, and the results suggest that the immunochromatographic test may be further improved for better investigation in asymptomatic dogs. The results obtained confirm the usefulness of DPP® CVL for application in serological surveys. | |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | Ministério da Saúde. Instituto Oswaldo Cruz | |
dc.rights | open access | |
dc.subject | Teste rápido CVL de plataforma de caminho duplo | |
dc.subject | Leishmaniasis | |
dc.subject | Dual-path platform CVL rapid test | |
dc.subject | Diagnosis | |
dc.subject | Dogs | |
dc.subject | Leishmaniasis Visceral | |
dc.subject | Perros | |
dc.subject | Leishmaniose Visceral | |
dc.subject | Diagnóstico | |
dc.subject | Cães | |
dc.title | Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis | |
dc.type | Article | |