dc.creatorFigueiredo, Fabiano Borges
dc.creatorVasconcelos, Tassia Cristina Bello de
dc.creatorMadeira, Maria de Fátima
dc.creatorMenezes, Rodrigo Caldas
dc.creatorMaia-Elkhoury, Ana Nilce Silveira
dc.creatorMarcelino, Andreza Pain
dc.creatorWerneck, Guilherme L.
dc.date2019-06-28T19:44:44Z
dc.date2019-06-28T19:44:44Z
dc.date2018
dc.date.accessioned2023-09-26T23:34:13Z
dc.date.available2023-09-26T23:34:13Z
dc.identifierFIGUEIREDO, Fabiano Borges et al. Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 11, p. 1-7, 2018.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33735
dc.identifier10.1590/0074-02760180260
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891987
dc.descriptionVisceral leishmaniasis is a major public health challenge in South America, and dogs are its main urban reservoir. It has the objective to do the validation of the canine Dual-path Platform immunoassay for canine visceral leishmaniasis (DPP® CVL) for a sample set composed of 1446 dogs from different Brazilian endemic areas. It had as methods a well-defined reference standard by means of parasitological culture, immunohistochemistry, and histopathology was used. Animals were classified as asymptomatic, oligosymptomatic, or symptomatic. Sensitivity and specificity were assessed as a single set and in clinical groups. A reproducibility assessment of the tests was conducted using the Kappa (κ) index at three different laboratories (A, B, and C). It was findings that overall, 89% sensitivity and 70% specificity were obtained for the entire sample set. Analysis of the clinical groups showed a gradual decrease in the sensitivity and an increase in the specificity with the reduction of clinical signs in the dogs that were assessed, reaching a sensitivity of 75% (42.8-94.5%) among asymptomatic dogs and lower specificity of 56% (46.2-66.3%) among symptomatic dogs. Inter-laboratory agreement was substantial (κAB= 0.778; κAC= 0.645; κCB= 0.711). The main conclusion was that the test performance is somewhat dependent on canine symptomatology, but such influence was less evident than in previous studies. Favourable results for sensitivity and specificity can be obtained even in asymptomatic animals; however, caution is needed in these evaluations, and the results suggest that the immunochromatographic test may be further improved for better investigation in asymptomatic dogs. The results obtained confirm the usefulness of DPP® CVL for application in serological surveys.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMinistério da Saúde. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectTeste rápido CVL de plataforma de caminho duplo
dc.subjectLeishmaniasis
dc.subjectDual-path platform CVL rapid test
dc.subjectDiagnosis
dc.subjectDogs
dc.subjectLeishmaniasis Visceral
dc.subjectPerros
dc.subjectLeishmaniose Visceral
dc.subjectDiagnóstico
dc.subjectCães
dc.titleValidation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis
dc.typeArticle


Este ítem pertenece a la siguiente institución