Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species

dc.creatorFróes, Adriana M.
dc.creatorSantos, Cláudia V. D. dos
dc.creatorPenha Filho, Manoel Leoncio da
dc.creatorTeixeira, Márcia Cristina Aquino
dc.creatorSilva, Tânia Maria Correia
dc.creatorOliveira, Geraldo Gileno de Sá
dc.creatordosSantos, Washington Luis Conrado
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorNeves, Neuza Maria Alcântara
dc.date2014-05-28T17:32:26Z
dc.date2014-05-28T17:32:26Z
dc.date2004
dc.date.accessioned2023-09-26T23:34:11Z
dc.date.available2023-09-26T23:34:11Z
dc.identifierFROES, Adriana M. et al. Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species. Veterinary Immunology and Immunopathology, v. 99, n. 3-4, p. 135–141, 2004.
dc.identifier0165-2427
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7662
dc.identifier10.1016/j.vetimm.2004.01.013
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891982
dc.descriptionThis work aims at identifying an effective protocol to raise anti-Leishmania chagasi amastigote antibodies in different animal species. Protocols of immunization by subcutaneous injections of L. chagasi promastigote and amastigote lysates or by either intravenous or subcutaneous inoculation of live metacyclic promastigotes were assessed in mice, rabbits, and dogs. The immunization with live promastigotes produced a strong humoral immune response against L. chagasi amastigotes in all three animal species. The sera from animals immunized with the promastigote lysate did not react with amastigotes and, conversely, the sera from mice immunized with the amastigote lysate did not react with promastigotes. Taken all data together, the immunization through infection with metacyclic promastigotes was considered the most satisfactory way to immunize animals for obtaining anti-amastigote and anti-promastigote antibodies, since it did not only allowed the obtention of antibody against the two forms of the parasite, but it is also cheap, less laborious than carrying out the purification of amastigotes from infected tissues and avoid the use of a large number of hamsters for obtention the amastigotes, necessary to produce the immunogenic lysates. Furthermore, this immunization protocol was comparable to the amastigote lysate immunization protocol for the obtaining of mouse monoclonal antibodies (mAbs).
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectImmune response
dc.subjectLeishmania chagasi-amastigotes
dc.subjectAntibodies-monoclonal
dc.subjectImmunohistology
dc.subjectDoenças do Cão/imunologia
dc.subjectImunização/veterinária
dc.subjectLeishmania/imunologia
dc.subjectLeishmaniose Cutânea/imunologia
dc.subjectCoelhos/parasitologia
dc.subjectDoenças dos Roedores/imunologia
dc.subjectAnticorpos Monoclonais/imunologia
dc.subjectAnimais
dc.subjectAntígenos de Protozoários/imunologia
dc.subjectCricetinae
dc.subjectDoenças do Cão/parasitologia
dc.subjectCães
dc.subjectHibridomas/imunologia
dc.subjectImunização/métodos
dc.subjectInjeções Intravenosas/veterinária
dc.subjectLeishmaniose Cutânea/parasitologia
dc.subjectCamundongos
dc.subjectLeishmaniose Cutânea/prevenção & controle
dc.subjectDoenças dos Roedores/parasitologia
dc.titleSub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
dc.typeArticle


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