Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.

dc.creatorToledo, Juliano Simões de
dc.creatorSantos, André Filipe Junqueira dos
dc.creatorMoura, Tatiana Rodrigues de
dc.creatorAntoniazi, Simone Aparecida
dc.creatorBrodskyn, Claudia Ida
dc.creatorOliveira, Camila Indiani de
dc.creatorBarral, Aldina Maria Prado
dc.creatorCruz, Angela Kaysel
dc.date2014-09-16T14:23:08Z
dc.date2014-09-16T14:23:08Z
dc.date2009
dc.date.accessioned2023-09-26T23:33:24Z
dc.date.available2023-09-26T23:33:24Z
dc.identifierTOLEDO, J. S. et al. Leishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo. Parasitology International, v. 58, n. 1, p. 45-50, 2009.
dc.identifier1383-5769
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8387
dc.identifier10.1016/j.parint.2008.09.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891842
dc.descriptionThe miniexon gene has a central role in the processing of polycistronic pre-mRNA of kinetoplastids. It is added to the 5′ extremity of each mRNA, supplying the 5′-capped structure to the molecule. Previous studies in Leishmania (Leishmania) major showed that the overexpression of the miniexon array attenuates the virulence of the parasite in in vivo assays. The results presented here extend those findings to Viannia subgenus. Leishmania (Viannia) braziliensis was transfected with a cosmid harboring a tandem array of one hundred miniexon gene copies and then characterized by Northern blot analysis. The overexpression of the exogenous gene was confirmed and its effect on the virulence of L. (V.) braziliensis was investigated in hamsters. In BALB/c mice we could not detect parasites during the course of 15 weeks of infection. In addition, hamsters infected with transfectants overexpressing the miniexon gene exhibited only a minor footpad swelling of late onset and failed to develop progressive lesion, these attenuated parasites could be recovered from the inoculation site 1 year after infection. The persistence of parasites in the host indicates that a stable line overexpressing the miniexon may be tested as live vaccine against leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Ltd
dc.rightsopen access
dc.subjectLeishmania (V.) braziliensis
dc.subjectVirulence
dc.subjectMiniexon
dc.subjectOverexpression
dc.subjectSL RNA
dc.titleLeishmania (Viannia) braziliensis transfectants overexpressing the miniexon gene lose virulence in vivo.
dc.typeArticle


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