The effect of glycerol on signal supression during electrospray ionization analysis of proteins

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMendes, M. A.
dc.creatorSouza, B. M.
dc.creatorMarques, M. R.
dc.creatorPalma, Mario Sergio
dc.date2014-05-27T11:21:05Z
dc.date2016-10-25T18:19:39Z
dc.date2014-05-27T11:21:05Z
dc.date2016-10-25T18:19:39Z
dc.date2004-06-04
dc.date.accessioned2017-04-06T01:09:27Z
dc.date.available2017-04-06T01:09:27Z
dc.identifierSpectroscopy, v. 18, n. 2, p. 339-345, 2004.
dc.identifier0712-4813
dc.identifierhttp://hdl.handle.net/11449/67763
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67763
dc.identifier10.1155/2004/732938
dc.identifierWOS:000221869800024
dc.identifier2-s2.0-2442641392.pdf
dc.identifier2-s2.0-2442641392
dc.identifierhttp://dx.doi.org/10.1155/2004/732938
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889178
dc.descriptionThe effect of salts, detergents and chaotropic agents on mass spectrometric analysis are relatively well understood, mainly due to their actions decreasing the performance of ESI interface in mass spectrometric analysis. However, there are few studies in the literature characterizing the effect of protein stabilization by glycerol, followed in some circumstances by the suppression of protein signal when ESI interface is used. The aim of the present research was to investigate in details the mass spectrometric behavior of some proteins in presence of high levels of glycerol during ESI-MS analysis. Thus, horse heart myoglobin and chicken ovalbumin were used as standard proteins. It was demonstrated that the presence of 1% (v/v) glycerol suppressed the signal of these proteins during the ESI-MS analysis, even when the sample nozzle potential was scanned from 28 to 80 V. However, when the glycerol concentration was decreased to 0.5% (v/v) and the sample cone voltage adjusted to 50 V, a perfect envelope of peaks was observed, allowing the spectrum deconvolution and the molecular mass determination with mass accuracy lower than 0.01% in each situation. A molecular explanation for this suppressive effect and for the analytical overcoming of this difficult is proposed.
dc.languageeng
dc.relationSpectroscopy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCone voltage
dc.subjectElectrospray ionization (ESI)
dc.subjectSignal supression
dc.subjectBiomedical engineering
dc.subjectDetergents
dc.subjectIonization
dc.subjectMass spectrometry
dc.subjectProteins
dc.subjectGlycerol
dc.subjectglycerol
dc.subjectmyoglobin
dc.subjectovalbumin
dc.subjectchicken
dc.subjectconference paper
dc.subjectcontrolled study
dc.subjectelectric potential
dc.subjectelectrospray
dc.subjecthorse
dc.subjectmass spectrometry
dc.subjectnonhuman
dc.subjectprotein analysis
dc.subjectsignal transduction
dc.subjectstandardization
dc.titleThe effect of glycerol on signal supression during electrospray ionization analysis of proteins
dc.typeOtro


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