A simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania species.

dc.creatorTeixeira, Márcia Cristina Aquino
dc.creatorSantos, Regilene de Jesus
dc.creatorSampaio, Romina Barreto
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatordosSantos, Washington Luis Conrado
dc.date2012-07-19T19:26:04Z
dc.date2012-07-19T19:26:04Z
dc.date2002
dc.date.accessioned2023-09-26T23:31:44Z
dc.date.available2023-09-26T23:31:44Z
dc.identifierTEIXEIRA, M.C. et al. A simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania species. Parasitology Research, v. 88, n. 11, p. 963-8, Nov. 2002.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/4212
dc.identifierDOI 10.1007/s00436-002-0695-3
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891543
dc.descriptionThis work describes a simple method to yield large amounts of Leishmania amastigote-like forms in axenic cultures using promastigotes as the starting population. The method described induced extracellular amastigote transformation of Leishmania amazonensis (97%), Leishmania braziliensis (98%) and Leishmania chagasi (90%). The rounded parasites obtained in axenic cultures were morphologically similar, even at the ultrastructural level, to intracellular amastigotes. Moreover, the axenic amastigotes remained viable as measured by their ability to revert back to promastigotes and to infect BALB/c mice. L. amazonensis and L. braziliensis promastigotes and axenic amastigotes differed in terms of their Western blot profiles. A 46 kDa protein was recognized by specific antibodies only in axenic and lesion-derived L. amazonensis amastigotes and not in promastigotes.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectLeishmania/crescimento & desenvolvimento
dc.subjectParasitologia/métodos
dc.subjectAnimais
dc.subjectWestern Blotting
dc.subjectCricetinae
dc.subjectMeios de Cultura
dc.subjectHumanos
dc.subjectLeishmania/patogenicidade
dc.subjectLeishmania/ultraestrutura
dc.subjectLeishmaniose/parasitologia
dc.subjectLeishmaniose/fisiopatologia
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectMicroscopia Eletrônica
dc.subjectReprodutibilidade dos Testes
dc.titleA simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania species.
dc.typeArticle


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