The resistance of the cowpea cv. BRS Xiquexique to infestation by cowpea weevil is related to the presence of toxic chitin-binding proteins

dc.creatorFerreira, Sarah Rodrigues
dc.creatorRocha, Maurisrael de Moura
dc.creatorSilva, Kaesel Jackson Damasceno
dc.creatorFerreira, Andre T. S.
dc.creatorPerales, Jonas
dc.creatorFernandes, Kátia V. S.
dc.creatorOliveira, Antonia E. A.
dc.date2021-11-05T12:48:32Z
dc.date2021-11-05T12:48:32Z
dc.date2021
dc.date.accessioned2023-09-26T23:31:16Z
dc.date.available2023-09-26T23:31:16Z
dc.identifierFERREIRA, Sarah Rodrigues et al. The resistance of the cowpea cv. BRS Xiquexique to infestation by cowpea weevil is related to the presence of toxic chitin-binding proteins. Pesticide Biochemistry and Physiology, v. 173, p. 1-9, 21 Jan. 2021.
dc.identifier0048-3575
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/49702
dc.identifier10.1016/j.pestbp.2021.104782
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891456
dc.descriptionThe cowpea weevil (Callosobruchus maculatus) is the main pest that attacks cowpea (Vigna unguiculata) seeds during storage, causing nutritional and economic losses in the cowpea crop. Thus, studies aiming to identify resistant cowpea cultivars have been developed. Chitin-binding proteins (CBP), such vicilins and chitinases, have been detected in seeds and related with the toxicity to insects. In this work, we investigated the presence of chitin-binding proteins in the partially resistant cowpea cv. BRS Xiquexique and evaluated their toxicity towards cowpea weevil. The CBP fraction was isolated by chitin affinity chromatography. CBP fraction showed, through 15% SDS PAGE, protein bands with varying molecular masses, mainly below 55 kDa. Proteins present in CBP fraction were identified by Western blotting and mass spectrometry analysis, as vicilins and chitinases. CBP fraction, at 5%, was able to interfere with the development of cowpea weevil, decreasing larval mass and length. A CBV (chitin-binding vicilin) fraction isolated from CBP fraction was toxic, at 2.0%, to C. maculatus, decreasing larval mass and length in 64.3% and 33.23%, respectively. These results suggest that chitin binding proteins, such vicilins and chitinases, may be related to the resistance of cowpea cv. BRS Xiquexique to the infestation by C. maculatus.
dc.description2023
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectVigna unguiculata
dc.subjectCallosobruchus maculatus
dc.subjectCultivares resistentes
dc.subjectProteínas de defesa
dc.subjectQuitinase
dc.subjectVicilins
dc.subjectVigna unguiculata
dc.subjectCallosobruchus maculatus
dc.subjectResistant cultivars
dc.subjectDefense proteins
dc.subjectChitinase
dc.subjectVicilins
dc.titleThe resistance of the cowpea cv. BRS Xiquexique to infestation by cowpea weevil is related to the presence of toxic chitin-binding proteins
dc.typeArticle


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