The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells

dc.creatorPinto, Mauro Cunha Xavier
dc.creatorCota, Betania Barros
dc.creatorRodrigues, Michele A.
dc.creatorLeite, Maria Fatima
dc.creatorFagundes, Elaine Maria Souza
dc.date2018-11-19T12:11:55Z
dc.date2018-11-19T12:11:55Z
dc.date2013
dc.date.accessioned2023-09-26T23:31:00Z
dc.date.available2023-09-26T23:31:00Z
dc.identifierPINTO, Mauro Cunha Xavier et al. The Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells. J Biochem Mol Toxicol., v. 27, n. 11, p. 479-485, 2013
dc.identifier1095-6670
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30086
dc.identifier10.1002/jbt.21507
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891410
dc.descriptionHypnophilin (HNP) is a sesquiterpene that is isolated from Lentinus cf. strigosus and has cytotoxic activities. Here, we studied the calcium signaling and cytotoxic effects ofHNPinUACC-62cells, ahuman skin melanoma cell line. HNP was able to increase the intracellular calcium concentration in UACC-62 cells, which was blocked in cells stimulated in Ca2+-freemedia. HNP treatment with BAPTA-AM, an intracellular Ca2+ chelator, caused an increase in calcium signals. HNP showed cytotoxicity against UACC-62 cells in which it induced DNA fragmentation and morphological alterations, including changes in the nuclear chromatin profile and increased cytoplasmatic vacuolization, but it had no effect on the plasma membrane integrity. These data suggest that cytotoxicity in UACC-62 cells, after treatment with HNP, is associated with Ca2+ influx. Together, these findings suggest that HNP is a relevant tool for the further investigation of new anticancer approaches.
dc.description2100-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsrestricted access
dc.subjectprodutos naturais
dc.subjectcelulas cancerigenas
dc.subjectNatural Products
dc.subjectCancer Cells
dc.subjectApoptosis
dc.subjectCalcium Signaling
dc.titleThe Cytotoxic and Proapoptotic Activities of Hypnophilin are Associated with Calcium Signaling in UACC-62 Cells
dc.typeArticle


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