An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil

dc.creatorSilva, A. M. V.
dc.creatorLeite, E. G.
dc.creatorAssis, R. M. S.
dc.creatorMajerowicz, S.
dc.creatorLeite, José Paulo G.
dc.date2020-10-24T14:19:03Z
dc.date2020-10-24T14:19:03Z
dc.date2001
dc.date.accessioned2023-09-26T23:30:55Z
dc.date.available2023-09-26T23:30:55Z
dc.identifierSILVA, A. M. V. et al. An Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 8, p. 1069-1073, Nov. 2001.
dc.identifier0074-0206
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44161
dc.identifier10.1590/S0074-02762001000800007
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891396
dc.descriptionBetween June 4th and June 20th 1996 rotavirus, adenovirus, and astrovirus (HAstrV) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the Bertha Lutz day care center, in Rio de Janeiro. All fecal samples were analyzed by polyacrylamide gel electrophoresis (PAGE), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme immunoassays (EIA), and electron microscopy (EM). Nine of them (33%) showed positive results for HAstrV by at least one of the employed methodologies. Eight were positive by RT-PCR and EIA, and six by EM. All positive samples were inoculated onto HT-29 (human colon adenocarcinoma) cultured cells for HAstrV isolation and seven were positive after three passages. The sequencing analysis of eight RT-PCR products (449 bp) from gene that codifies VP2 protein, showed a total nucleotide identity among them and 98% with HAstrV-1 (strain Oxford type 1). This is the first report of a gastroenteritis outbreak associated with HAstrv-1 in a day care center in Rio de Janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectAstrovirus
dc.subjectGastroenterite
dc.subjectSurto
dc.subjectRio de Janeiro
dc.subjectAstrovirus
dc.subjectGastroenteritis -
dc.subjectOutbreak
dc.subjectRio de Janeiro
dc.subjectBrazil
dc.titleAn Outbreak of Gastroenteritis Associated with Astrovirus Serotype 1 in a Day Care Center, in Rio de Janeiro, Brazil
dc.typeArticle


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