A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.

dc.creatorJesus, Joílson Ramos de
dc.creatorCarvalho, Kellyanne dos Anjos
dc.creatorFonseca, Rosana Anita da Silva
dc.creatorOliveira, Geraldo Gileno de Sá
dc.creatorMelo, Stella Maria Barrouin
dc.creatorNeves, Neuza Maria Alcântara
dc.creatorDutra, Rosa Amalia Fireman
dc.date2014-09-25T17:28:33Z
dc.date2014-09-25T17:28:33Z
dc.date2011
dc.date.accessioned2023-09-26T23:30:53Z
dc.date.available2023-09-26T23:30:53Z
dc.identifierRAMOS-JESUS, J. et al. A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum. Analytical and Bioanalytical Chemistry, v. 401, n. 3, p. 917-925, 2011.
dc.identifier1618-2650
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8448
dc.identifier10.1007/s00216-011-5136-7
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891390
dc.descriptionThe American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was developed. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaraldehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen-antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.rightsopen access
dc.subjectLeishmania chagasi
dc.subjectImmunosensor
dc.subjectSelf-assembled monolayer
dc.subjectPiezoelectric
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectBioensaio/instrumentação
dc.subjectLeishmaniose Visceral/sangue
dc.subjectAnimais
dc.subjectAntígenos/química
dc.subjectCães
dc.subjectEletrodos
dc.subjectLeishmania infantum/imunologia
dc.subjectLeishmaniose Visceral/imunologia
dc.subjectSistemas Micro-Eletromecânicos/métodos
dc.subjectQuartzo
dc.titleA piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
dc.typeArticle


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