Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis

dc.creatorSantos, Lenita Ramires dos
dc.creatorMelo, Stella Maria Barrouin
dc.creatorChang, Yung-Fu
dc.creatorOlsen, John
dc.creatorMcDonough, Sean P
dc.creatorQuimby, Fred
dc.creatordosSantos, Washington Luis Conrado
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorOliveira, Geraldo Gileno de Sá
dc.date2014-09-08T12:00:17Z
dc.date2014-09-08T12:00:17Z
dc.date2004
dc.date.accessioned2023-09-26T23:30:43Z
dc.date.available2023-09-26T23:30:43Z
dc.identifierSANTOS, enita Ramires dos et al. Recombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis. Veterinary Immunology Immunopathology, v. 98, n. 1-2, p. 43-48, 2004.
dc.identifier0165-2427
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8309
dc.identifier10.1016/j.vetimm.2003.10.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891357
dc.descriptionCanine visceral leishmaniasis poses important concerns for public health and veterinary medicine in many areas of the world. Resistance to it seems to be associated with cellular specific immune responses of the so-called Th1 type. Interleukin-12 (IL-12) is one of the most potent inducers of Th1 type of immune responses to co-administered antigens. Herein, the cloning of canine IL-12, as a single-chain fusion protein (sccaIL-12), and its expression in biologically active form in COS-7 cells is reported. Supernatants from these cells stimulated the expression of comparable amounts of interferon gamma mRNA in peripheral blood mononuclear cells from dogs with natural visceral leishmaniasis. In addition, after stimulation with sccaIL-12, there was no difference between interferon gamma mRNA expressions in peripheral blood mononuclear cells of dogs with visceral leishmaniasis and from normal healthy control animals.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier B.V
dc.rightsrestricted access
dc.subjectCanine recombinant IL-12
dc.subjectVisceral leishmaniasis
dc.subjectGamma interferon expression
dc.subjectDoenças do Cão/quimioterapia
dc.subjectIndutores de Interferon/farmacologia
dc.subjectInterleucina-12/farmacologia
dc.subjectInterferon gama/genética
dc.subjectLeishmaniose Visceral/veterinária
dc.subjectRNA Mensageiro/genética
dc.subjectAnimais
dc.subjectSequência de Bases
dc.subjectClonagem Molecular
dc.subjectDoenças do Cão/genética
dc.subjectCães
dc.subjectExpressão Gênica
dc.subjectIndutores de Interferon/química
dc.subjectInterleucina-12/química
dc.subjectLeishmaniose Visceral/quimioterapia
dc.subjectLeucócitos Mononucleares/efeitos de drogas
dc.subjectProteínas Recombinantes/química
dc.subjectProteínas Recombinantes/farmacologia
dc.titleRecombinant single-chain canine interleukin 12 induces interferon gamma mRNA expression in peripheral blood mononuclear cells of dogs with visceral leishmaniasis
dc.typeArticle


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