Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease

dc.creatorNhantumbo, Aquino Albino
dc.creatorCantarelli, Vlademir Vicente
dc.creatorCaireão, Juliana
dc.creatorMunguambe, Alcides Moniz
dc.creatorComé, Charlotte Elizabeth
dc.creatorPinto, Gabriela do Carmo
dc.creatorZimba, Tomás Francisco
dc.creatorMandomando, Inácio
dc.creatorSemá, Cynthia Baltazar
dc.creatorDias, Cícero
dc.creatorMoraes, Milton Ozório
dc.creatorGudo, Eduardo Samo
dc.date2016-03-29T14:10:33Z
dc.date2016-03-29T14:10:33Z
dc.date2015
dc.date.accessioned2023-09-26T23:30:27Z
dc.date.available2023-09-26T23:30:27Z
dc.identifierNHANTUMBO, Aquino Albino; et al. Frequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease. Plos One, v.10, n.9, e0138249, Sept. 2015.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13383
dc.identifier10.1371/journal.pone.0138249
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891308
dc.descriptionBACKGROUND: In Sub-Saharan Africa, including Mozambique, acute bacterial meningitis (ABM) represents a main cause of childhood mortality. The burden of ABM is seriously underestimated because of the poor performance of culture sampling, the primary method of ABM surveillance in the region. Low quality cerebrospinal fluid (CSF) samples and frequent consumption of antibiotics prior to sample collection lead to a high rate of false-negative results. To our knowledge, this study is the first to determine the frequency of ABM in Mozambique using real-time polymerase chain reaction (qPCR) and to compare results to those of culture sampling. METHOD: Between March 2013 and March 2014, CSF samples were collected at 3 regional hospitals from patients under 5 years of age, who met World Health Organization case definition criteria for ABM. Macroscopic examination, cytochemical study, culture, and qPCR were performed on all samples. RESULTS: A total of 369 CSF samples were collected from children clinically suspected of ABM. qPCR showed a significantly higher detection rate of ABM-causing pathogens when compared to culture (52.3% [193/369] versus 7.3% [27/369], p = 0.000). The frequency of Streptococcus pneumoniae, Haemophilus influenzae, group B Streptococci, and Neisseria meningitidis were 32.8% (121⁄369), 12.2%, (45⁄369), 3.0% (16⁄369) and 4.3% (11⁄369), respectively, significantly higher compared to that obtained on culture (p < 0.001 for each). CONCLUSION: Our findings demonstrate that culture is less effective for the diagnosis of ABM than qPCR. The common use of culture rather than qPCR to identify ABM results in serious underestimation of the burden of the disease, and our findings strongly suggest that qPCR should be incorporated into surveillance activities for ABM. In addition, our data showed that S. pneumoniae represents the most common cause of ABM in children under 5 years of age.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectMeningitis
dc.subjectMozambique
dc.subjectBurden of Disease
dc.subjectMultiplex Polymerase Chain Reaction
dc.subjectMeningite
dc.subjectEfeitos Psicossociais da Doença
dc.subjectReação em Cadeia da Polimerase Multiplex
dc.subjectMoçambique
dc.titleFrequency of Pathogenic Paediatric Bacterial Meningitis in Mozambique: The Critical Role of Multiplex Real-Time Polymerase Chain Reaction to Estimate the Burden of Disease
dc.typeArticle


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