Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates

dc.creatorSilva, Pollyanna Michelle da
dc.creatorBaldry, Mara
dc.creatorPeng, Pai
dc.creatorSilva, Juliane Nancy de Oliveira
dc.creatorSoares, Tatiana
dc.creatorBrayner, Fábio André
dc.creatorAlves, Luiz Carlos
dc.creatorFeitosa, Ana Paula Sampaio
dc.creatorPaiva, Patrícia Maria Guedes
dc.creatorIngmer, Hanne
dc.creatorNapoleão, Thiago Henrique
dc.date2019-05-24T15:02:48Z
dc.date2019-05-24T15:02:48Z
dc.date2019
dc.date.accessioned2023-09-26T23:30:15Z
dc.date.available2023-09-26T23:30:15Z
dc.identifierSILVA, Pollyanna Michelle da et al. Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates. International Journal of Biological Macromolecules, v. 123, p. 600–608, 15 Feb. 2019.
dc.identifier1879-0003
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33212
dc.identifier10.1016/j.ijbiomac.2018.11.030
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891272
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) pelas bolsas de pesquisa (446902 / 2014-4) e bolsas de estudo (PMGP e THN) e à Fampaupode Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) pelo apoio financeiro (APQ). -0108-2.08 / 14; APQ-0661-2.08 / 15). O PMS gostaria de agradecer à FACEPE pela bolsa de pós-graduação (IBPG-0467-2.08 / 14) e à COORDENAÇÃO DE Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Código de Finanças 001) pela bolsa de doutorado sanduíche.
dc.descriptionIn this work, we evaluated the ability of Punica granatum sarcotesta lectin (PgTeL) to impair the growth and viability of the Staphylococcus aureus clinical isolates 8325-4 (non-resistant) and LAC USA300 (MRSA strain). The effects of this lectin on aggregating, hemolytic activity, biofilm-forming ability, and expression of virulence genes (hla, rnaIII, and spa) were also investigated. PgTeL showed antibacterial activity against 8325-4 and LAC USA300 strains by interfering with both the growth (MIC50 of 6.25 and 12.5 μg/mL, respectively) and survival (MBC values of 25.0 and 50.0 μg/mL, respectively). Culture growth started only at the ninth (8325-4) and tenth (LAC USA300) hour in the presence of PgTeL at MIC50, while growth was detected since the first hour in the control. The lectin caused markedly altered cell morphology in both the strains. Although, at the MIC50, PgTeL caused structural alterations, most cells were still viable, while at the MBC it promoted cell injury and death. PgTeL showed anti-aggregation effect and exhibited antibiofilm activity against both the isolates. However, the lectin did not interfere with the hemolytic activity of LAC USA300 and with the expression of hla, rnaIII, and spa genes. In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectAntibacterial activity
dc.subjectFruit lectin
dc.subjectMRSA isolate
dc.subjectPomegranate
dc.subjectAgr system
dc.subjectBiofilmes / efeitos de drogas
dc.subjectBiofilmes / crescimento e desenvolvimento
dc.subjectAgregação Celular / efeitos de drogas
dc.subjectProliferação Celular / efeitos de drogas
dc.subjectSobrevivência Celular / efeitos de drogas
dc.subjectHumanos
dc.subjectLectinas / química
dc.subjectLectinas / farmacologia
dc.subjectStaphylococcus aureus / efeitos de drogas
dc.subjectStaphylococcus aureus / patogenicidade
dc.titlePunica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates
dc.typeArticle


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