Characterization of Leishmania species from Central-West Region of Brazil

dc.creatorCastro, Ludiele Souza
dc.creatorFrança, Adriana de Oliveira
dc.creatorFerreira, Eduardo de Castro
dc.creatorLima Júnior, Manoel Sebastião da Costa
dc.creatorGontijo, Célia Maria Ferreira
dc.creatorPereira, Agnes Antônia Sampaio
dc.creatorDorval, Maria Elizabeth Cavalheiros
dc.date2019-05-02T15:33:13Z
dc.date2019-05-02T15:33:13Z
dc.date2018
dc.date.accessioned2023-09-26T23:29:33Z
dc.date.available2023-09-26T23:29:33Z
dc.identifierCASTRO, Ludiele Souza et al. Characterization of Leishmania Species from Central-West Region of Brazil. Parasitology Research, v. 117, n. 6, p. 1839-1845, 2018.
dc.identifier1432-1955
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32913
dc.identifier10.1007/s00436-018-5871-1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891155
dc.descriptionLeishmaniasis represents a complex of diseases with a broad clinical spectrum and epidemiological diversity. Involving a variety of agents, reservoirs, and vectors, cutaneous leishmaniasis (CL) presents different transmission patterns, which makes its control especially difficult. Epidemiological characterization with identification of existing Leishmania species in a particular region is essential for understanding this parasitosis epidemiology. Because similar results may be observed in places with overlapping transmission of visceral and cutaneous leishmaniasis, contributing to knowledge about the epidemiological cycle of cutaneous leishmaniasis is of global interest. This study proposes to identify the different species of Leishmania present in human CL cases in the state of Mato Grosso do Sul, Brazil. Slides of imprint tissue were selected from patients diagnosed with CL from 2009 to 2013 at the Laboratório de Parasitologia Clínica, Universidade Federal de Mato Grosso do Sul. The slides were scraped and extracted genetic material was amplified with PCR addressing the Leishmania spp. kDNA region. The reaction product was subjected to RFLP for the specific identification of Leishmania. Forty-three samples (82.7%) amplified with PCR were considered positive. Of these, it was possible to determine the species in 40 samples (76.9%) through analysis of electrophoresis profiles. Leishmania (Viannia) braziliensis, Leishmania (Leishmania) amazonensis, and Leishmania (Leishmania) infantum were identified as the etiologic agents of CL in Mato Grosso do Sul. The results of this study permit a more detailed understanding of CL epidemiology and the geographical distribution of its agents.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCutaneous leishmaniasis
dc.subjectSpecies identification
dc.subjectLeishmania
dc.subjectAdulto
dc.subjectAnimais
dc.subjectBrasil / epidemiologia
dc.subjectDNA, cinetoplasto / genética
dc.subjectEletroforese
dc.subjectFêmea
dc.subjectHumanos
dc.subjectLeishmania braziliensis / genética
dc.subjectLeishmania braziliensis / isolamento & purificação
dc.subjectLeishmania infantum / genética
dc.subjectLeishmania infantum / isolamento & purificação
dc.subjectLeishmaniose Cutânea / epidemiologia
dc.subjectLeishmaniose cutânea / parasitologia
dc.subjectLeishmaniose cutânea / transmissão
dc.subjectLeishmaniose Visceral / epidemiologia
dc.subjectLeishmaniose Visceral / parasitologia
dc.subjectLeishmaniose Visceral / transmissão
dc.subjectMasculino
dc.subjectMeia idade
dc.subjectReação em Cadeia da Polimerase
dc.subjectPolimorfismo, comprimento do fragmento de restrição
dc.titleCharacterization of Leishmania species from Central-West Region of Brazil
dc.typeArticle


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