The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA

dc.creatorBarbedo, Leonardo Silva
dc.creatorFigueiredo-Carvalho, Maria Helena Galdino
dc.creatorMuniz, Mauro de Medeiros
dc.creatorZancopé-Oliveira, Rosely Maria
dc.date2019-01-24T12:38:38Z
dc.date2019-01-24T12:38:38Z
dc.date2016
dc.date.accessioned2023-09-26T23:29:18Z
dc.date.available2023-09-26T23:29:18Z
dc.identifierBARBEDO, Leonardo Silva et al. The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 4, p. 267-270, Apr. 2016.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31282
dc.identifier10.1590/0074-02760150466
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891109
dc.descriptionCurrently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Oswaldo Cruz
dc.rightsopen access
dc.subjectCandida parapsilosis complex
dc.subjectPCR-RFLP
dc.subjectFungaemia
dc.titleThe identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA
dc.typeArticle


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