In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13

dc.creatorCoêlho, Zirlane Castelo Branco
dc.creatorTeixeira, Maria Jânia
dc.creatorMota, Erika Freitas
dc.creatorFrutuoso, Mércia Sindeaux
dc.creatorSilva, João Santana da
dc.creatorBarral, Aldina Maria Prado
dc.creatorBarral Netto, Manoel
dc.creatorPompeu, Margarida Maria de Lima
dc.date2014-03-12T19:12:11Z
dc.date2014-03-12T19:12:11Z
dc.date2010
dc.date.accessioned2023-09-26T23:28:59Z
dc.date.available2023-09-26T23:28:59Z
dc.identifierCOELHO, Z. C. B. et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. Brazilian Journal of Infectious Diseases, v. 14, n. 5, p.472-482, 2010.
dc.identifier1678-4391
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7399
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8891048
dc.descriptionThe initial encounter of Leishmania with its host's immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profile and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV's PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in first hours after Leishmania stimulation, with the definition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Editora Ltda
dc.rightsopen access
dc.subjectLeishmania amazonensis
dc.subjectInterleukin-10
dc.subjectInterleukin-13
dc.subjectInterações Hospedeiro-Parasita/imunologia
dc.subjectImunidade Humoral/imunologia
dc.subjectInterleucina-10/biossíntese
dc.subjectInterleucina-13/biossíntese
dc.subjectLeishmania mexicana/imunologia
dc.subjectLeucócitos Mononucleares/parasitologia
dc.subjectCitocinas/biossíntese
dc.subjectDNA de Protozoário/análise
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectHumanos
dc.subjectInterleucina-10/imunologia
dc.subjectInterleucina-13/imunologia
dc.subjectLeishmania mexicana/fisiologia
dc.subjectLeucócitos Mononucleares/imunologia
dc.subjectRNA de Protozoário/análise
dc.subjectReação em Cadeia da Polimerase Via Transcriptase Reversa
dc.titleIn vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
dc.typeArticle


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