Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice.

dc.creatorDaltro, Pâmela Santana
dc.creatorAlves, Paula Santana
dc.creatorCastro, Murilo Fagundes de
dc.creatorAzevedo, Carine Machado
dc.creatorVasconcelos, Juliana Fraga
dc.creatorAllahdadi, Kyan James
dc.creatorFreitas, Luiz Antonio Rodrigues de
dc.creatorSouza, Bruno Solano de Freitas
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorSoares, Milena Botelho Pereira
dc.creatorMacambira, Simone Garcia
dc.date2016-03-23T16:47:24Z
dc.date2016-03-23T16:47:24Z
dc.date2015
dc.date.accessioned2023-09-26T23:27:42Z
dc.date.available2023-09-26T23:27:42Z
dc.identifierDALTRO, P. S. et al. Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice. BMC Cardiovascular Disorders, v. 15, p. 162, 2015.
dc.identifier1471-2261
dc.identifier10.1186/s12872-015-0154-6
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13255
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890815
dc.descriptionBackground/Objectives: High fat diet (HFD) is a major contributor to the development of obesity and cardiovascular diseases due to the induction of cardiac structural and hemodynamic abnormalities. We used a model of diabetic cardiomyopathy in C57Bl/6 mice fed with a HFD to investigate the effects of granulocyte-colony stimulating factor (G-CSF), a cytokine known for its beneficial effects in the heart, on cardiac anatomical and functional abnormalities associated with obesity and type 2 diabetes. Methods: Groups of C57Bl/6 mice were fed with standard diet (n = 8) or HFD (n = 16). After 36 weeks, HFD animals were divided into a group treated with G-CSF + standard diet (n = 8) and a vehicle control group + standard diet (n = 8). Cardiac structure and function were assessed by electrocardiography, echocardiography and treadmill tests, in addition to the evaluation of body weight, fasting glicemia, insulin and glucose tolerance at different time points. Histological analyses were performed in the heart tissue. Results: HFD consumption induced metabolic alterations characteristic of type 2 diabetes and obesity, as well as cardiac fibrosis and reduced exercise capacity. Upon returning to a standard diet, obese mice body weight returned to non-obese levels. G-CSF administration accelerated the reduction in of body weight in obese mice. Additionally, G-CSF treatment reduced insulin levels, diminished heart fibrosis, increased exercise capacity and reversed cardiac alterations, including bradycardia, elevated QRS amplitude, augmented P amplitude, increased septal wall thickness, left ventricular posterior thickening and cardiac output reduction. Conclusion: Our results indicate that G-CSF administration caused beneficial effects on obesity-associated cardiac impairment.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectDiabetes
dc.subjectDiabetic cardiomyopathy
dc.subjectObesity
dc.subjectG-CSF
dc.subjectDiabetes Mellitus Tipo 2/complicações
dc.subjectCardiomiopatias Diabéticas/quimioterapia
dc.subjectFator Estimulador de Colônias de Granulócitos/uso terapêutico
dc.subjectObesidade/complicações
dc.subjectAdiponectina/sangue
dc.subjectAnimais
dc.subjectGlicemia/metabolismo
dc.subjectColesterol/sangue
dc.subjectDiabetes Mellitus Tipo 2/patologia
dc.subjectCardiomiopatias Diabéticas/patologia
dc.subjectDieta Hiperlipídica
dc.subjectModelos Animais de Doenças
dc.subjectFibrose
dc.subjectHemodinâmica
dc.subjectInsulina/sangue
dc.subjectMasculino
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectObesidade/fisiopatologia
dc.titleAdministration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice.
dc.typeArticle


Este ítem pertenece a la siguiente institución