Plasmodium simium, a Plasmodium vivax-Related Malaria Parasite: Genetic Variability of Duffy Binding Protein II and the Duffy Antigen/Receptor for Chemokines.

dc.creatorCosta, Daniela Camargos
dc.creatorAssis, Gabriela Maíra Pereira de
dc.creatorSilva, Flávia Alessandra de Souza
dc.creatorAraújo, Flávia Carolina
dc.creatorSouza Junior, Júlio César de
dc.creatorHirano, Zelinda Maria Braga
dc.creatorKano, Flora Satiko
dc.creatorSousa, Taís Nóbrega de
dc.creatorCarvalho, Luzia Helena
dc.creatorBrito, Cristiana Ferreira Alves de
dc.date2016-02-16T18:00:04Z
dc.date2016-02-16T18:00:04Z
dc.date2015
dc.date.accessioned2023-09-26T23:27:29Z
dc.date.available2023-09-26T23:27:29Z
dc.identifierCOSTA, Daniela Camargos et al. Plasmodium simium, a Plasmodium vivax-Related Malaria Parasite: Genetic Variability of Duffy Binding Protein II and the Duffy Antigen/Receptor for Chemokines. PLoS ONE 10(6): e0131339
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12741
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890775
dc.descriptionPlasmodium simium is a parasite from New World monkeys that is most closely related to the human malaria parasite Plasmodium vivax; it also naturally infects humans. The blood-stage infection of P. vivax depends on Duffy binding protein II (PvDBPII) and its cognate receptor on erythrocytes, the Duffy antigen receptor for chemokines (hDARC), but there is no information on the P. simium erythrocytic invasion pathway. The genes encoding P. simium DBP (PsDBPII) and simian DARC (sDARC) were sequenced from Southern brown howler monkeys (Alouatta guariba clamitans) naturally infected with P. simium because P. simium may also depend on the DBPII/DARC interaction. The sequences of DBP binding domains from P. vivaxand P. simium were highly similar. However, the genetic variability of PsDBPII was lower than that of PvDBPII. Phylogenetic analyses demonstrated that these genes were strictly related and clustered in the same clade of the evolutionary tree. DARC from A. clamitans was also sequenced and contained three new non-synonymous substitutions. None of these substitutions were located in the N-terminal domain of DARC, which interacts directly with DBPII. The interaction between sDARC and PvDBPII was evaluated using a cytoadherence assay of COS7 cells expressing PvDBPII on their surfaces. Inhibitory binding assays in vitrodemonstrated that antibodies from monkey sera blocked the interaction between COS-7 cells expressing PvDBPII and hDARC-positive erythrocytes. Taken together, phylogenetic analyses reinforced the hypothesis that the host switch from humans to monkeys may have occurred very recently in evolution, which sheds light on the evolutionary history of new world plasmodia. Further invasion studies would confirm whether P. simium depends on DBP/DARC to trigger internalization into red blood cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectPlasmodium
dc.subjectRed blood cells
dc.subjectSequence alignment
dc.subjectMalaria
dc.subjectEnzyme-linked immunoassays
dc.subjectMalarial parasites
dc.subjectHaplotypes
dc.titlePlasmodium simium, a Plasmodium vivax-Related Malaria Parasite: Genetic Variability of Duffy Binding Protein II and the Duffy Antigen/Receptor for Chemokines.
dc.typeArticle


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