Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens

dc.creatorSantos, Emily Ferreira dos
dc.creatorLeony, Leonardo Maia
dc.creatorSilva, Ângelo Antônio Oliveira
dc.creatorDaltro, Ramona Tavares
dc.creatorFreitas, Natália Erdens Maron de
dc.creatorVasconcelos, Larissa de Carvalho Medrado
dc.creatorAraújo, Fernando Luiz Vieira de
dc.creatorCeledon, Paola Alejandra Fiorani
dc.creatorKrieger, Marco Aurélio
dc.creatorZanchin, Nilson Ivo Tonin
dc.creatorSantos, Fred Luciano Neves
dc.date2021-08-11T17:08:32Z
dc.date2021-08-11T17:08:32Z
dc.date2021
dc.date.accessioned2023-09-26T23:27:24Z
dc.date.available2023-09-26T23:27:24Z
dc.identifierSANTOS, Emily Ferreira dos et al. Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens. Transfusion, v. 61, n. 9, p. 2701-2709, Sept. 2021.
dc.identifier0041-1132
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48579
dc.identifier10.1111/trf.16583
dc.identifier1537-2995
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890759
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnologico, Grant/Award Numbers: 309263/2020-4, 312195/2015-0, 590032/2011-9
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior, Grant/Award Number: 001
dc.descriptionFundação de Amparo à Pesquisa do Estado da Bahia, Grant/Award Numbers: BOL0543/2020, BOL0932/2020
dc.descriptionInova Fiocruz/VPPCB, Grant/Award Number: VPPCB-008-FIO-18-2-20
dc.descriptionBackground: Chagas disease (CD) serological screening at blood banks is usually performed by a single highly sensitive serological assay, with chemiluminescent immunoassays (CLIAs) being the method of choice. CLIAs employ recombinant, fusion peptides and/or chimeric antigens that selectively capture anti-Trypanosoma cruzi antibodies. However, despite high sensitivity, the ability of these tests to identify CD-positive cases should be evaluated against T. cruzi strains circulating in specific locales. Herein, we used a latent class analysis (LCA) approach employing an array of four chimeric antigens to assess the diagnostic performance of the Liaison XL Murex Chagas CLIA for the detection of anti-T. cruzi IgG in serum samples. Study Design and Methods: The study included a panel of 5014 serum samples collected from volunteer blood donors at the Hematology and Hemotherapy Foundation of the State of Bahia, submitted to anti-T. cruzi antibody detection using Liaison Chagas CLIA and LCA as a reference test in the absence of a gold standard. Results: LCA classified 4993 samples as negative, while positivity for T. cruzi antibodies was predicted in 21 samples. Compared with LCA, CLIA demonstrated sensitivity and specificity of 76.2% and 99.5%, respectively, providing an overall accuracy of 99.4%. Discussion: In blood banks lacking a de facto highly sensitive screening immunoassay, the low sensitivity offered by Liaison Chagas CLIA renders it unsuitable for standalone use in serological screening procedures for CD. Moreover, blood banks are encouraged to carefully assess the ability of diagnostic methods to identify local T. cruzi strains in circulation.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectDoença de Chagas
dc.subjectLuminescência
dc.subjectImunoensaio
dc.subjectAnálise de classes latentes
dc.subjectTriagem sorológica
dc.subjectChagas disease
dc.subjectChemiluminescence
dc.subjectImmunoassay
dc.subjectLatent class analysis
dc.subjectSerological screening
dc.subjectEnfermedad de Chagas
dc.subjectLuminiscencia
dc.subjectInmunoensayo
dc.subjectAnálisis de Clases Latentes
dc.subjectSerología
dc.subjectMaladie de Chagas
dc.subjectLuminescence
dc.subjectDosage immunologique
dc.subjectAnalyse de structure latente
dc.subjectSérologie
dc.subjectDoença de Chagas
dc.subjectLuminescência
dc.subjectImunoensaio
dc.subjectAnálise de Classes Latentes
dc.subjectSorologia
dc.titleAssessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens
dc.typeArticle


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