An Sau3AI restriction endonuclease isoschizomer from Bacillus cereus

dc.creatorCruz, A. K.
dc.creatorKidane, G.
dc.creatorPires, M. Q.
dc.creatorRabinovitch, L.
dc.creatorGuaycurus, T. V.
dc.creatorMorel, Carlos Medicis
dc.date2016-06-28T14:12:10Z
dc.date2016-07-07T12:14:12Z
dc.date2016-06-28T14:12:10Z
dc.date2016-07-07T12:14:12Z
dc.date1984
dc.date.accessioned2023-09-26T23:27:17Z
dc.date.available2023-09-26T23:27:17Z
dc.identifierCRUZ, A. K. et al. An Sau3AI restriction endonuclease isoschizomer from Bacillus cereus. FEBS Letters, v.173, n.1, p.99-102, July 1984.
dc.identifier0014-5793
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14676
dc.identifier1873-3468
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890737
dc.descriptionThe isolation and characterization of a restriction endonuclease from Bacillus cereus IOC 243 are described. The enzyme recognizes the palindromic sequence 5’-G(met-A,A)TC-3’ as determined by PEI chromatography of pancreatic DNase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and N6-methyladenine-free DNA and direct sequence analysis of cloned fragments. The staggered cleavage products with 5 ‘-terminal pGATC extensions are efficiently labelled with polynucleotide kinase and are easily cloned into BamHI sites. The enzyme, denoted Bce243, is thus an isoschizomer of SaufAl. Its use and potential advantages in substituting Sau3AI are discussed.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectBacillus
dc.subjectRestriction endonuclease
dc.subjectMolecular cloning
dc.subjectSau3A
dc.subjectIsoschizomer
dc.subjectBacillus
dc.subjectClonagem Molecular
dc.titleAn Sau3AI restriction endonuclease isoschizomer from Bacillus cereus
dc.typeArticle


Este ítem pertenece a la siguiente institución