In vitro penetration of bleaching agents into the pulp chamber

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBenetti, A. R.
dc.creatorValera, M. C.
dc.creatorMancini, M. N G
dc.creatorMiranda, C. B.
dc.creatorBalducci, I.
dc.date2014-05-27T11:21:02Z
dc.date2016-10-25T18:19:22Z
dc.date2014-05-27T11:21:02Z
dc.date2016-10-25T18:19:22Z
dc.date2004-02-01
dc.date.accessioned2017-04-06T01:08:28Z
dc.date.available2017-04-06T01:08:28Z
dc.identifierInternational Endodontic Journal, v. 37, n. 2, p. 120-124, 2004.
dc.identifier0143-2885
dc.identifierhttp://hdl.handle.net/11449/67641
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67641
dc.identifier10.1111/j.0143-2885.2004.00761.x
dc.identifierWOS:000188822200004
dc.identifier2-s2.0-1342343189
dc.identifierhttp://dx.doi.org/10.1111/j.0143-2885.2004.00761.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889070
dc.descriptionAim: To investigate pulp chamber penetration of bleaching agents in teeth following restorative procedures. Methodology: Bovine lateral incisors were sectioned 3 mm apical to the cemento-enamel junction and the coronal pulpal tissue was removed. Teeth were divided into six groups (n = 10): G1, G2 and G3 were not submitted to any restorative procedure, while G4, G5 and G6 were submitted to Class V preparations and restored with composite resin. Acetate buffer was placed in the pulp chamber and treatment agents were applied for 60 min at 37°C as follows: G1 and G4, immersion into distilled water; G2 and G5, 10% carbamide peroxide (CP) exposure; G3 and G6, 35% CP bleaching. The buffer solution was removed and transferred to a glass tube where leuco crystal violet and horseradish peroxidase were added, producing a blue solution. The optical density of the blue solution was determined spectrophotometrically at 596 nm. A standard curve made with known amounts of hydrogen peroxide was used to convert the optical density values of the coloured samples into microgram equivalents of hydrogen peroxide. Data were submitted to ANOVA and Tukey's test (5%). Results: Amounts of hydrogen peroxide found in the pulp chamber of G2 and G5 specimens (0.1833 ± 0.2003 μg) were significantly lower (P = 0.001) when compared to G3 and G6 specimens (0.4604 ± 0.3981 μg). Restored teeth held significantly higher (P = 0.001) hydrogen peroxide concentrations in the pulp chamber than intact teeth. Conclusion: Higher concentrations of the bleaching agent produced higher levels of hydrogen peroxide in the pulp chamber, especially in restored teeth.
dc.languageeng
dc.relationInternational Endodontic Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarbamide peroxide
dc.subjectDental bleaching
dc.subjectPulp
dc.subjectacetic acid
dc.subjectbleaching agent
dc.subjectbuffer
dc.subjectcrystal violet
dc.subjectglass
dc.subjecthorseradish peroxidase
dc.subjecthydrogen peroxide
dc.subjectperoxide
dc.subjectresin
dc.subjectunclassified drug
dc.subjecturea derivative
dc.subjecturea peroxide
dc.subjectwater
dc.subjectanalysis of variance
dc.subjectanimal tissue
dc.subjectcattle
dc.subjectcontrolled study
dc.subjectexposure
dc.subjectin vitro study
dc.subjectincisor
dc.subjectnonhuman
dc.subjectoptical density
dc.subjectreparative dentistry
dc.subjectspectrophotometry
dc.subjecttissue section
dc.subjecttooth crown
dc.subjecttooth filling
dc.subjecttooth pulp
dc.subjecttooth root
dc.subjecttube
dc.subjectwater immersion
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectCattle
dc.subjectComposite Resins
dc.subjectDental Enamel Permeability
dc.subjectDental Pulp Cavity
dc.subjectDental Restoration, Permanent
dc.subjectDrug Combinations
dc.subjectHydrogen Peroxide
dc.subjectOxidants
dc.subjectPeroxides
dc.subjectStatistics, Nonparametric
dc.subjectTooth Bleaching
dc.subjectTooth Crown
dc.subjectUrea
dc.titleIn vitro penetration of bleaching agents into the pulp chamber
dc.typeOtro


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