IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells

dc.creatorVan Weyenbergh, Johan Jozef Rosa Maria
dc.creatorSilva, Maria da Purificação Pereira da
dc.creatorBafica, Andre Luiz Barbosa
dc.creatorCardoso, Silvia
dc.creatorWietzerbin, Juana
dc.creatorBarral Netto, Manoel
dc.date2015-06-30T16:31:52Z
dc.date2015-06-30T16:31:52Z
dc.date2001
dc.date.accessioned2023-09-26T23:26:43Z
dc.date.available2023-09-26T23:26:43Z
dc.identifierVAN WEYENBERGH, Johan Jozef Rosa Maria et al. IFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells. Immunology Letters, v. 75, n. 2, p. 117-122, 2001.
dc.identifier0165-2478
dc.identifierS0165-2478(00)00303-5
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11017
dc.identifier10.1016/S0165-2478(00)00303-5
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890635
dc.descriptionBoth IFN-b and TGF-b have demonstrated their ability to antagonize several of the stimulatory activities of IFN-g on human macrophages, thereby classifying them as Th2-like. Aiming at a further characterization of their role in Th1:Th2 development, we studied their possible interaction with IL-12, the key Th1 cytokine. We found that IFN-b by itself induced modest amounts of IFN-g, but was able to synergize with IL-12 for IFN-g induction. TGF-b, on the other hand, had no effect by itself and inhibited significantly the IL-12-induced IFN-g secretion. The differential effect of IFN-b and TGF-b on IL-12 bioactivity was most pronounced upon IFN-g synthesis, since IFN-b induced only marginal amounts of IL-10 and IL-12 and TGF-b diminished constitutive IL-10 production, while neither had a significant effect on TNF-a production. Although monocytes did not produce detectable IFN-g with any of the stimuli, adherent cells were found to cooperate with non-adherent lymphocytes for maximal IFN-g production. However, IL-18, a monocyte-derived IFN-g-inducing cytokine able to synergize with IL-12, was undetectable in IFN-b or IFN-b IL-12-stimulated cells. In conclusion, the ability of IFN-b to synergize with IL-12 for IFN-g synthesis, without significant concomitant IL-10 production, suggest a strong boost to Th1 development, which seems to be IL-18-independent.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectIFN-g
dc.subjectSynergy
dc.subjectAntagonism
dc.subjectLeishmaniasis
dc.subjectMultiple sclerosis
dc.subjectInterferon Tipo I/farmacologia
dc.subjectInterleucina-12/metabolismo
dc.subjectLeucócitos Mononucleares/efeitos de drogas
dc.subjectLeucócitos Mononucleares/imunologia
dc.subjectFator de Crescimento Transformador beta/farmacologia
dc.subjectAdesão Celular
dc.subjectInterações de Medicamentos
dc.subjectTécnicas In Vitro
dc.subjectHumanos
dc.subjectInterferon Tipo I/administração & dosagem
dc.subjectInterferon gama/biossíntese
dc.subjectInterleucina-12/administração & dosagem
dc.subjectLeucócitos Mononucleares/citologia
dc.subjectProteínas Recombinantes
dc.subjectCélulas Th1/efeitos de drogas
dc.subjectFator de Crescimento Transformador beta/administração & dosagem
dc.subjectFator de Necrose Tumoral alfa/biossíntese
dc.titleIFN-beta and TGF-beta differentially regulate IL-12 activity in human peripheral blood mononuclear cells
dc.typeArticle


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