Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil

dc.creatorArruda, Sérgio Marcos
dc.creatorChalhoub, Marcelo
dc.creatorCardoso, Silvia Andrade
dc.creatorBarral Netto, Manoel
dc.date2014-03-25T17:46:14Z
dc.date2014-03-25T17:46:14Z
dc.date1998
dc.date.accessioned2023-09-26T23:25:53Z
dc.date.available2023-09-26T23:25:53Z
dc.identifierARRUDA, S. et al. Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil. Acta Tropica, v. 71, p. 1-15, 1998.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7445
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890495
dc.descriptionEvaluating human immune response to defined Mycobacterium tuberculosis antigens in patients with different clinical forms of tuberculosis may help in elucidating pathogenesis and in vaccine development. In the present report we evaluated the lymphocyte proliferation, cytokine production and natural killer cell cytotoxicity as parameters to screen four mycobacterial recombinant antigens. Pleural fluid mononuclear cells (PFMC) and peripheral blood mononuclear cells (PBMC) from 13 HIV-negative patients with tuberculous pleurisy, living in a tropical region of Brazil were used in these assays. Crude M. tuberculosis antigen and recombinant 70-, 65- and 38-kDa mycobacterial antigens, induced greater proliferation in PFMC than in PBMC. IFN-gamma, TNF-alpha, IL-4 and IL-10 were evaluated in the PFMC supernatants stimulated by these antigens. Both crude and 70-kDa antigens induced higher levels of IFN-gamma, TNF-alpha and IL-10. There was a significant positive correlation between IFN-gamma and the proliferative response induced by crude M. tuberculosis antigen, and an inverse correlation was identified between IL-10 and cell proliferation. IL-4 was not detected in the supernatants of pleural fluid mononuclear cell cultures stimulated by either crude, or recombinant antigens. TNF-alpha was detected in variable amounts in supernatants of PFMC stimulated by all antigens tested. Natural killer cytotoxicity was induced by both crude and 70-kDa antigen. Our results demonstrate that cells present at the site of disease recognized three of the antigens screened, as shown by lymphocyte proliferation and production of regulatory and inflammatory cytokines, and the results obtained with PFMC were consistently higher than those obtained with homologous PBMC.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Science B.V
dc.rightsopen access
dc.subjectAntígenos de Bactérias/imunologia
dc.subjectCitocinas/sangue
dc.subjectCitotoxicidade Imunológica/imunologia
dc.subjectAtivação Linfocitária/imunologia
dc.subjectMycobacterium tuberculosis/imunologia
dc.subjectTuberculose Pleural/imunologia
dc.subjectAnticorpos Monoclonais
dc.subjectCitotoxicidade Imunológica/fisiologia
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectHumanos
dc.subjectImunidade Celular/imunologia
dc.subjectInterferon gama/sangue
dc.subjectInterleucina-10/sangue
dc.subjectCélulas Matadoras Naturais/imunologia
dc.subjectDerrame Pleural/imunologia
dc.subjectProteínas Recombinantes/imunologia
dc.subjectTuberculose Pleural/microbiologia
dc.subjectFator de Necrose Tumoral alfa/imunologia
dc.titleCell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
dc.typeArticle


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