Oral Outbreak of Chagas Disease in Santa Catarina, Brazil: Experimental Evaluation of a Patient’s Strain

dc.creatorDomingues, Carolina S.
dc.creatorHardoim, Daiana de Jesus
dc.creatorSouza, Celeste S. F.
dc.creatorCardoso, Flávia O.
dc.creatorMendes, Verônica G.
dc.creatorSilva, Henrique Previtalli
dc.creatorSilva, Ana L. Abreu
dc.creatorMachado, Marcelo Pelajo
dc.creatorCosta, Sylvio Celso Gonçalves da
dc.creatorCalabrese, Kátia S.
dc.date2015-11-24T23:07:26Z
dc.date2015-11-24T23:07:26Z
dc.date2015
dc.date.accessioned2023-09-26T23:25:46Z
dc.date.available2023-09-26T23:25:46Z
dc.identifierDOMINGUES, Carolina S. et al. Oral Outbreak of Chagas Disease in Santa Catarina, Brazil: Experimental Evaluation of a Patient’s Strain. Plos One, v.10, n.10, e0122566, 18p, 2015.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12317
dc.identifier10.1371/journal.pone.0122566
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890475
dc.descriptionChagas disease is a worldwide public health problem. Although the vectorial transmission of Chagas disease has been controlled in Brazil there are other ways of transmission, such as the ingestion of T. cruzi contaminated food, which ensures the continuation of this zoonosis. Here, we demonstrate the influence of the inoculation route on the establishment and development of the SC2005 T. cruzi strain infection in mice. Groups of Swiss mice were infected intragastrically (IG) or intraperitoneally (IP) with the T. cruzi SC2005 strain derived from an outbreak of oral Chagas disease. The results revealed that 100% of IP infected mice showed parasitemia, while just 36% of IG infected showed the presence of the parasite in blood. The parasitemia peaks were later and less intense in the IG infected mice. Mortality of the IP infected animals was more intense and earlier when compared to the IG infected mice. In the IP infected mice leucopenia occurred in the early infection followed by leucocytosis, correlating positively with the increase of the parasites. However, in the IG infected mice only an increase in monocytes was observed, which was positively correlated with the increase of the parasites. Histopathological analyses revealed a myotropic pattern of the SC2005 strain with the presence of inflammatory infiltrates and parasites in different organs of the animals infected by both routes as well as fibrosis foci and collagen redistribution. The flow cytometric analysis demonstrated a fluctuation of the T lymphocyte population in the blood, spleen and mesenteric lymph nodes of the infected animals. T. cruzi DNA associated with the presence of inflammatory infiltrates was detected by PCR in the esophagus, stomach and intestine of all infected mice. These findings are important for the understanding of the pathogenesis of T. cruzi infection by both inoculation routes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlos One
dc.rightsopen access
dc.subjectSanta Catarina
dc.subjectSurto Bucal
dc.subjectComida contaminada
dc.subjectChagas Disease
dc.subjectPublic Health
dc.subjectBrazil
dc.subjectTrypanosama cruzi
dc.subjectContaminated food
dc.subjectDoença de Chagas
dc.subjectSaúde Pública
dc.subjectTrypanosoma cruzi
dc.titleOral Outbreak of Chagas Disease in Santa Catarina, Brazil: Experimental Evaluation of a Patient’s Strain
dc.typeArticle


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