Experimental hepatitis A virus (HAV) infection in cynomolgus monkeys (Macaca fascicularis): evidence of active extrahepatic site of HAV replication

dc.creatorAmado, Luciane A.
dc.creatorMarchevsky, Renato S.
dc.creatorPaula, Vanessa S. de
dc.creatorHooper, Cleber
dc.creatorFreire, Marcos da S.
dc.creatorGaspar, Ana Maria C.
dc.creatorPinto, Marcelo A.
dc.date2018-12-08T19:27:49Z
dc.date2018-12-08T19:27:49Z
dc.date2010
dc.date.accessioned2023-09-26T23:25:45Z
dc.date.available2023-09-26T23:25:45Z
dc.identifierAMADO, Luciane A. et al. Experimental hepatitis A virus (HAV) infection in cynomolgus monkeys (Macaca fascicularis): evidence of active extrahepatic site of HAV replication. International Journal of Experimental Pathology, v. 91, p.87-97, Feb. 2010.
dc.identifier0959-9673
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30440
dc.identifier10.1111/j.1365-2613.2009.00699.x
dc.identifier1365-2613
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890472
dc.descriptionThis work studied the replication sites of hepatitis A virus (HAV) in cynomolgus monkeys (Macaca fascicularis) after intravenous inoculation. The cynomolgus monkeys were inoculated with the Brazilian hepatitis A virus strain (HAF-203). Monkeys were euthanized on days 15, 30, 45 and 60 postinoculation (pi). Liver samples, submandibular salivary gland, mesenteric lymph node and tonsils were removed for virological and pathological evaluation. Immunofluorescence analyses on liver and salivary gland sections using confocal laser scanning microscopy revealed the presence of HAV antigen (HAV Ag). The presence of HAV genome was monitored by real-time PCR. The HAV RNA was detected at 7 days postinoculation (dpi), concomitantly in serum, saliva and faeces. The highest HAV viral load was observed in faeces at 15 dpi (105 copies⁄ml), followed by serum viral load of 104 copies⁄ml at 20 dpi and saliva viral load of 103 copies⁄ml at 7 dpi. The animals showed first histological and biochemical signs of hepatitis at 15 dpi. The HAV antigen (Ag) was present from day 7 until day 60 pi in the liver and salivary glands. The HAV replicative intermediate was also detected in the liver (4.5 · 104 copies⁄mg), salivary glands (1.9 · 103 copies⁄mg), tonsils (4.2 · 101 copies⁄mg) and lymph nodes (3.4 · 101 copies⁄mg). Our data demonstrated that the salivary gland as an extrahepatic site of early HAV replication could create a potential risk of saliva transmitted infection. In addition, the cynomolgus monkey was confirmed as a suitable model to study the pathogenesis of HAV human infection.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsrestricted access
dc.subjectLocal extra-hepático
dc.subjectReplicativo intermediário
dc.subjectHepatite A
dc.subjectSaliva e patogênese
dc.subjectMacaca fascicularis
dc.subjectCynomolgus
dc.subjectExtrahepatic site
dc.subjectHepatitis A
dc.subjectIntermediate replicative
dc.subjectSaliva and pathogenesis
dc.titleExperimental hepatitis A virus (HAV) infection in cynomolgus monkeys (Macaca fascicularis): evidence of active extrahepatic site of HAV replication
dc.typeArticle


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