Molecular findings from influenza A(H1N1)pdm09 detected in patients from a Brazilian equatorial region during the pandemic period

dc.creatorOliveira, Maria José Couto
dc.creatorMotta, Fernando do Couto
dc.creatorSiqueira, Marilda Agudo Mendonça Teixeira de
dc.creatorResende, Paola Cristina
dc.creatorBorn, Priscilla da Silva
dc.creatorSouza, Thiago Moreno L
dc.creatorMesquita, Milene
dc.creatorOliveira, Maria de Lourdes Aguiar
dc.creatorCarney, Sharon
dc.creatorMello, Wyller Alencar de
dc.creatorMagalhães, Vera
dc.date2015-06-17T12:05:24Z
dc.date2015-06-17T12:05:24Z
dc.date2014
dc.date.accessioned2023-09-26T23:25:42Z
dc.date.available2023-09-26T23:25:42Z
dc.identifierOLIVEIRA, Maria José Couto et al.Molecular findings from influenza A(H1N1)pdm09 detected in patients from a Brazilian equatorial region during the pandemic period. Mem Inst Oswaldo Cruz, v.109, n.7, p.912-917, Nov. 2014.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10878
dc.identifier10.1590/0074-0276140210
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890463
dc.descriptionAfter the World Health Organization officially declared the end of the first pandemic of the XXI century in August 2010, the influenza A(H1N1)pdm09 virus has been disseminated in the human population. In spite of its sustained circulation, very little on phylogenetic data or oseltamivir (OST) resistance is available for the virus in equatorial regions of South America. In order to shed more light on this topic, we analysed the haemagglutinin (HA) and neuraminidase (NA) genes of influenza A(H1N1)pdm09 positive samples collected during the pandemic period in the Pernambuco (PE), a northeastern Brazilian state. Complete HA sequences were compared and amino acid changes were related to clinical outcome. In addition, the H275Y substitution in NA, associated with OST resistance, was investigated by pyrosequencing. Samples from PE were grouped in phylogenetic clades 6 and 7, being clustered together with sequences from South and Southeast Brazil. The D222N/G HA gene mutation, associated with severity, was found in one deceased patient that was pregnant. Additionally, the HA mutation K308E, which appeared in Brazil in 2010 and was only detected worldwide the following year, was identified in samples from hospitalised cases. The resistance marker H275Y was not identified in samples tested. However, broader studies are needed to establish the real frequency of resistance in this Brazilian region.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectInfluenza A(H1N1)pdm09
dc.subjectHaemagglutinin
dc.subjectVírus da Influenza A Subtipo H1N1
dc.subjectPyrosequencing
dc.subjectVírus da Influenza A Subtipo H1N1
dc.subjectOseltamivir
dc.subjectResistência a Medicamentos
dc.titleMolecular findings from influenza A(H1N1)pdm09 detected in patients from a Brazilian equatorial region during the pandemic period
dc.typeArticle


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