Molecular analysis of yellow fever virus 17DD vaccine strain

dc.creatorPost, Paulo R.
dc.creatorSantos, Claudia N. D.
dc.creatorCarvalho, Ricardo
dc.creatorLopes, Oscar S.
dc.creatorGaller, Ricardo
dc.date2020-09-17T18:47:00Z
dc.date2020-09-17T18:47:00Z
dc.date1991
dc.date.accessioned2023-09-26T23:24:34Z
dc.date.available2023-09-26T23:24:34Z
dc.identifierPOST, Paulo R. et al. Molecular analysis of yellow fever virus 17DD vaccine strain. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 2, p. 239-246, Apr./June 1991.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43443
dc.identifier10.1590/S0074-02761991000200015
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890263
dc.descriptionThe Oswaldo Cruz Foundation produces most of the yellow fever (YF) vaccine prepared world wide. As part of a broader approach to determine the genetic variability in YF l7D seeds and vaccines and its relevance to viral attenuation the 17DD virus was purifed directly from chick embryo homogenates which is the source of virus used for vaccination of millions of people in Brazil and other countries for half a century. Neutralization and hemagglutination tests showed that the purified virus is similar to the original stock. Furthermore, radioimmune precipitation of 35S-methionine-labeled viral proteins using mouse hyperimmune ascitic fluid revealed identical patterns for the purified 17DD virus and the YF l7D-204 strain except for the 17DD E protein which migrated slower on SDS-PAGE. This difference is likely to be due to N-linked glycosylation. Finally, comparison by northern blot nybridization of virion RNAs of purified 17DD with two other strains of YF virus only fenome-sized molecules for all three viruses. These observations suggest that vaccine phenotype is primarily associated with the accumulation of mutations.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectFebre Amarela
dc.subjectVacina
dc.subjectCepa 17DD
dc.subjectRNA
dc.subjectYellow Fever
dc.subjectVaccine
dc.subject17DD strain
dc.subjectEnvelope
dc.subjectRNA
dc.titleMolecular analysis of yellow fever virus 17DD vaccine strain
dc.typeArticle


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