Glucose homeostasis in pregnant rats submitted to dietary protein restriction

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRostom de Mello, Maria Alice
dc.creatorLuciano, Eliete
dc.creatorMagalhães Carneiro, Everardo
dc.creatorQueiroz Latorraca, Márcia
dc.creatorMachado de Oliveira, Camila Aparecida
dc.creatorBoschero, Antonio Carlos
dc.date2014-05-27T11:21:00Z
dc.date2016-10-25T18:19:15Z
dc.date2014-05-27T11:21:00Z
dc.date2016-10-25T18:19:15Z
dc.date2003-12-01
dc.date.accessioned2017-04-06T01:08:00Z
dc.date.available2017-04-06T01:08:00Z
dc.identifierResearch Communications in Molecular Pathology and Pharmacology, v. 113-114, p. 229-246.
dc.identifier1078-0297
dc.identifierhttp://hdl.handle.net/11449/67581
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67581
dc.identifier2-s2.0-13144266686
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889016
dc.descriptionIn the present work, we examined the effects of feeding a low protein diet during pregnancy on glucose-induced insulin secretion and glucose homeostasis in rats. Young (60 days), pregnant (P) or non-pregnant (NP) rats were fed during pregnancy or for 21 days (the NP) a normal (17%) or a low (6%) protein diet. Serum glucose and insulin levels and pancreas insulin content in the fed state; total area under serum glucose curve (AG) after a glucose load and serum glucose disappearance rate (Kitt) after insulin administration; as well as 86Rb outflow, 45Ca uptake and insulin secretion by isolated pancreatic islets in response to glucose were evaluated. Serum glucose was lower in 17%-P (12%) and 6%-P (27%) than in corresponding NP-rats. Serum insulin was higher in 17%- P (153%) and 6%-P (77%) compared to the corresponding NP-rats. Pancreatic insulin was higher in 6%-rats (55%) than in 17%-rats. No differences were found in AG among the groups whereas Kitt was lower in 6%-NP and higher in 6%-P than in the equivalent 17% rats. Increasing glucose concentration from 2.8 to 16.7 mmol/l, reduced 86Rb outflow from isolated islets from all groups. Increasing glucose concentration from 2.8 to 16.7 mmol/l elevated 45Ca uptake by 17%-NP (47%), 17%-P (40%) and 6%-P (214%) islets but not by 6%-NP ones. The increase in 45Ca uptake was followed by an increase in insulin release by the 17%-NP (2767%), 17%-P (2850%) and 6%-P (1200%) islets. In conclusion, 6%-P rats show impaired glucose induced insulin secretion related to reduced calcium uptake by pancreatic islets. However, the poor insulin secretion did not fully compensate the high peripheral sensitivity to the hormone, resulting in hypoglycemia.
dc.languageeng
dc.relationResearch Communications in Molecular Pathology and Pharmacology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject45Ca uptake
dc.subject86Rb outflow
dc.subjectGlucose metabolism
dc.subjectInsulin secretion
dc.subjectPancreatic islets
dc.subjectPregnancy
dc.subjectProtein malnutrition
dc.subjectRat
dc.subjectcalcium
dc.subjectglucose
dc.subjectrubidium
dc.subjectanimal experiment
dc.subjectcalcium transport
dc.subjectcontrolled study
dc.subjectglucose blood level
dc.subjectglucose homeostasis
dc.subjectglucose metabolism
dc.subjectinsulin blood level
dc.subjectinsulin release
dc.subjectnonhuman
dc.subjectpancreas islet
dc.subjectpregnancy
dc.subjectpriority journal
dc.subjectprotein diet
dc.subjectprotein restriction
dc.subjectrat
dc.subjectAnimals
dc.subjectCalcium
dc.subjectFatty Acids, Nonesterified
dc.subjectFemale
dc.subjectGlucose
dc.subjectHomeostasis
dc.subjectInsulin
dc.subjectIslets of Langerhans
dc.subjectLiver
dc.subjectProtein Deficiency
dc.subjectRats
dc.subjectRats, Wistar
dc.titleGlucose homeostasis in pregnant rats submitted to dietary protein restriction
dc.typeOtro


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