Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice

dc.creatorIborra, Salvador
dc.creatorParody, Nuria
dc.creatorAbánades, Daniel Ruiz
dc.creatorBonay, Pedro
dc.creatorPrates, Deboraci Brito
dc.creatorNovais, Fernanda Oliveira
dc.creatorBarral Netto, Manoel
dc.creatorAlonso, Carlos
dc.creatorSoto, Manuel
dc.date2014-03-13T19:19:59Z
dc.date2014-03-13T19:19:59Z
dc.date2008
dc.date.accessioned2023-09-26T23:23:46Z
dc.date.available2023-09-26T23:23:46Z
dc.identifierIBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008.
dc.identifier1286-4579
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7408
dc.identifier10.1016/j.micinf.2008.06.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890116
dc.descriptionIn the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice.We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-g response mediated mainly by CD4þ T cell, albeit a minor contribution of CD8þ T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-g production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine. 2008 Elsevier Masson SAS. All rights reserved
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Masson SAS
dc.rightsopen access
dc.subjectLeishmania
dc.subjectBALB/C mice
dc.subjectC57BL/6 mice
dc.subjectTh1/Th2 immune responses;
dc.subjectRibosomal proteins
dc.subjectVaccines
dc.subjectLeishmania major/imunologia
dc.subjectVacinas contra Leishmaniose
dc.subjectLeishmaniose Cutânea/prevenção & controle
dc.subjectOligodesoxirribonucleotídeos/imunologia
dc.subjectProteínas Ribossômicas/imunologia
dc.subjectAnimais
dc.subjectFeminino
dc.subjectImunoglobulina G/metabolismo
dc.subjectInterferon gama/metabolismo
dc.subjectInterleucina-10/metabolismo
dc.subjectInterleucina-4/metabolismo
dc.subjectLeishmaniose Cutânea/imunologia
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectOligodesoxirribonucleotídeos/genética
dc.subjectOligodesoxirribonucleotídeos/metabolismo
dc.subjectProteínas de Protozoários/genética
dc.subjectProteínas de Protozoários/imunologia
dc.subjectProteínas de Protozoários/metabolismo
dc.subjectProteínas Ribossômicas/genética
dc.subjectProteínas Ribossômicas/metabolismo
dc.titleVaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
dc.typeArticle


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