Inflammasome Genetic Variants Are Associated with Protection to Clinical Severity of COVID-19 among Patients from Rio de Janeiro, Brazil

dc.creatorSá, Nathalia Beatriz Ramos de
dc.creatorGoulart, Milena Neira
dc.creatorAlves, Marcelo Ribeiro
dc.creatorPerazzo, Hugo
dc.creatorGeraldo, Kim Mattos
dc.creatorRibeiro, Maria Pia Diniz
dc.creatorCardoso, Sandra Wagner
dc.creatorGrinsztejn, Beatriz
dc.creatorVeloso, Valdiléa G.
dc.creatorCapão, Artur
dc.creatorSiqueira, Marilda Mendonça
dc.creatorBezerra, Ohanna Cavalcanti de Lima
dc.creatorGarcia, Cristiana Couto
dc.creatorGomes, Larissa Rodrigues
dc.creatorCazote, Andressa da Silva
dc.creatorAlmeida, Dalziza Victalina de
dc.creatorGiacoia-Gripp, Carmem Beatriz Wagner
dc.creatorCôrtes, Fernanda Heloise
dc.creatorMorgado, Mariza Gonçalves
dc.date2022-10-11T12:06:48Z
dc.date2022-10-11T12:06:48Z
dc.date2022
dc.date.accessioned2023-09-26T23:23:35Z
dc.date.available2023-09-26T23:23:35Z
dc.identifierSÁ, Nathalia Beatriz Ramos de et al. Inflammasome Genetic Variants Are Associated with Protection to Clinical Severity of COVID-19 among Patients from Rio de Janeiro, Brazil. BioMed Research International, v.2022, Article 9082455, p. 1 - 15, 2022.
dc.identifier2314-6133
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55097
dc.identifier10.1155/2022/9082455
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8890083
dc.descriptionCOVID-19 has a broad spectrum of clinical manifestations, from asymptomatic or mild/moderate symptoms to severe symptoms and death. The mechanisms underlying its clinical evolution are still unclear. Upon SARS-CoV-2 infection, host factors, such as the inflammasome system, are activated by the presence of the virus inside host cells. The search for COVID-19 risk factors is of relevance for clinical management. In this study, we investigated the impact of inflammasome single-nucleotide polymorphisms (SNPs) in SARS-CoV-2-infected individuals with distinct severity profiles at clinical presentation. Patients were divided into two groups according to disease severity at clinical presentation based on the WHO Clinical Progression Scale. Group 1 included patients with mild/moderate disease (WHO< 6; n = 76), and group 2 included patients with severe/critical COVID-19 (WHO≥ 6; n = 357). Inpatients with moderate to severe/critical profiles were recruited and followed-up at Hospital Center for COVID-19 Pandemic – National Institute of Infectology (INI)/FIOCRUZ, RJ, Brazil, from June 2020 to March 2021. Patients with mild disease were recruited at Oswaldo Cruz Institute (IOC)/FIOCRUZ, RJ, Brazil, in August 2020. Genotyping of 11 inflammasome SNPs was determined by real-time PCR. Protection and risk estimation were performed using unconditional logistic regression models. Significant differences in NLRP3 rs1539019 and CARD8 rs2043211 were observed between the two groups. Protection against disease severity was associated with the A/A genotype (ORadj = 0:36; P = 0:032), allele A (ORadj = 0:93; P = 0:010), or carrier-A (ORadj = 0:45; P = 0:027) in the NLRP3 rs1539019 polymorphism; A/T genotype (ORadj = 0:5; P = 0:045), allele T (ORadj = 0:93; P = 0:018), or carrier-T (ORadj = 0:48; P = 0:029) in the CARD8 rs2043211 polymorphism; and the A-C-G-C-C (ORadj = 0:11; P = 0:018), A-C-G-C-G (ORadj = 0:23; P = 0:003), C-C-G-C-C (ORadj = 0:37; P = 0:021), and C-T-G-A-C (ORadj = 0:04; P = 0:0473) in NLRP3 genetic haplotype variants. No significant associations were observed for the other polymorphisms. To the best of our knowledge, this is the first study demonstrating an association between CARD8 and NLRP3 inflammasome genetic variants and protection against COVID-19 severity, contributing to the discussion of the impact of inflammasomes on COVID-19 outcomes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi
dc.rightsopen access
dc.subjectCOVID-19
dc.subjectVariantes genéticas do inflamassoma
dc.subjectProteção
dc.subjectGravidade clínica
dc.subjectPacientes
dc.subjectRio de Janeiro
dc.subjectCOVID-19
dc.subjectInflammasome Genetic Variants
dc.subjectProtection
dc.subjectClinical Severity of COVID-19
dc.subjectPatients
dc.subjectRio de Janeiro, Brazil
dc.titleInflammasome Genetic Variants Are Associated with Protection to Clinical Severity of COVID-19 among Patients from Rio de Janeiro, Brazil
dc.typeArticle


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