Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.

dc.creatorNeves, Neuza Maria Alcântara
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2014-09-09T16:56:31Z
dc.date2014-09-09T16:56:31Z
dc.date1995
dc.date.accessioned2023-09-26T23:23:09Z
dc.date.available2023-09-26T23:23:09Z
dc.identifierALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8334
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889999
dc.descriptionParasite-derived trans-sialidase (TS) activity was demonstrated in the serum and blood of Trypanosoma cruzi-infected mice. Serum TS activity levels correlated well with parasitemia in BALB/c and Swiss mice during the initial stages of the infection. However, in later stages the TS activity levels decreased despite increasing parasitemia. This coincided with the appearance of circulating TS antibodies. On the other hand, there was always a good correlation between TS activity and parasitemia in athymic nude mice. Sera from mice with high parasitemia and low TS activity inhibited TS activity in vitro. The inhibition was also observed with purified serum IgG, and it was absorbed by staphylococcal protein A, indicating that it was caused by anti-TS IgG antibodies. These antibodies inhibited the enzymatic activity of insolubilized TS, indicating that they act by interfering with the catalytic site rather than by aggregating the enzyme. The presence of inhibitory antibodies, however, did not prevent the progression of parasitemia in BALB/c mice.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag
dc.rightsopen access
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectDoença de Chagas /sangue
dc.subjectGlicoproteínas /sangue
dc.subjectNeuraminidase /sangue
dc.subjectTrypanosoma cruzi /enzimologia
dc.subjectAnimais
dc.subjectWestern Blotting
dc.subjectDoença de Chagas /enzimologia
dc.subjectEletroforese em Gel de Poliacrilamida
dc.subjectFeminino
dc.subjectGlicoproteínas /antagonistas & inibidores
dc.subjectImunoglobulina G /isolamento & purificação
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectNeuraminidase /antagonistas & inibidores
dc.subjectParasitemia /enzimologia
dc.subjectFatores de Tempo
dc.subjectTrypanosoma cruzi /imunologia
dc.titleCirculating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
dc.typeArticle


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