Effects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamine

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBarbisan, Luís Fernando
dc.creatorScolastici, Clarissa
dc.creatorMiyamoto, Maristela
dc.creatorFarero Salvadori, Daisy Maria
dc.creatorRibeiro, Lúcia Regina
dc.creatorFerreira Da Eira, Augusto
dc.creatorViana de Camargo, João Lauro
dc.date2014-05-27T11:20:59Z
dc.date2016-10-25T18:19:10Z
dc.date2014-05-27T11:20:59Z
dc.date2016-10-25T18:19:10Z
dc.date2003-12-01
dc.date.accessioned2017-04-06T01:07:41Z
dc.date.available2017-04-06T01:07:41Z
dc.identifierGenetics and Molecular Research, v. 2, n. 3, p. 295-308, 2003.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/67543
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67543
dc.identifier2-s2.0-2542421821.pdf
dc.identifier2-s2.0-2542421821
dc.identifierhttp://www.geneticsmr.com//year2003/vol2-3/pdf/gmr0056.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888979
dc.descriptionThe effects of crude extracts of the mushroom Agaricus blazei Murrill (Agaricaceae) on both DNA damage and placental form glutathione S-transferase (GST-P)-positive liver foci induced by diethylnitrosamine (DEN) were investigated. Six groups of adult male Wistar rats were used. For two weeks, animals of groups 3 to 6 were treated with three aqueous solutions of A. blazei (mean dry weight of solids being 1.2, 5.6, 11.5 and 11.5 mg/ml, respectively). After this period, groups 2 to 5 were given a single ip injection 200 mg/kg DEN and groups 1 and 6 were treated with 0.9% NaCl. All animals were subjected to 70% partial hepatectomy at week five and sacrificed 4, 24 and 48 h or 8 weeks after DEN or 0.9% NaCl treatments (10th week after the beginning of the experiment). The alkaline comet assay and GST-P-positive liver foci development were used to evaluate the influence of the mushroom extracts on liver cell DNA damage and on the initiation of liver carcinogenesis, respectively. Previous treatment with the highest concentration of A. blazei (11.5 mg/ml) significantly reduced DNA damage, indicating a protective effect against DEN-induced liver cytotoxicity/genotoxicity. However, the same dose of mushroom extract significantly increased the number of GST-P-positive liver foci.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAgaricux blazei
dc.subjectDNA damage
dc.subjectGST-P-positive liver foci
dc.subjectHepatocarcinogenesis
dc.subjectdiethylnitrosamine
dc.subjectglutathione transferase
dc.subjectliver protective agent
dc.subjectAgaricus blazei
dc.subjectanimal cell
dc.subjectanimal model
dc.subjectcomet assay
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjectfungus
dc.subjectgenotoxicity
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectliver carcinogenesis
dc.subjectliver cell
dc.subjectliver necrosis
dc.subjectnonhuman
dc.subjectpartial hepatectomy
dc.subjectrat
dc.subjecttail flick test
dc.subjectweight gain
dc.subjectAgaricaceae
dc.subjectAgaricus
dc.subjectAnimalia
dc.subjectBasidiomycota
dc.subjectFungi
dc.subjectRattus norvegicus
dc.titleEffects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamine
dc.typeOtro


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