Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil

dc.creatorSuzukawa, Andréia Akemi
dc.creatorZanluca, Camila
dc.creatorJorge, Natasha Andressa Nogueira
dc.creatorNoronha, Lucia de
dc.creatorKoishi, Andrea Cristine
dc.creatorPaula, Caroline B. V. de
dc.creatorRebutini, Patrícia Z.
dc.creatorNagashima, Seigo
dc.creatorHansel-Frose, Aruana F. F.
dc.creatorParreira, Vinícius S. C.
dc.creatorBordignon, Juliano
dc.creatorMacDonald, Margaret R.
dc.creatorRice, Charles M.
dc.creatorPassetti, Fabio
dc.creatorSantos, Claudia Nunes Duarte dos
dc.date2020-10-30T16:19:11Z
dc.date2020-10-30T16:19:11Z
dc.date2020
dc.date.accessioned2023-09-26T23:21:32Z
dc.date.available2023-09-26T23:21:32Z
dc.identifierSUZUKAWA, Andréia A. et al. Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil. Journal of Infection, p. 1-10, 2020.
dc.identifier0163-4453
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44273
dc.identifier10.1016/j.jinf.2020.09.028
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889702
dc.descriptionScreening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be involved in ZIKV congenital pathogenesis. Methods: Transcriptome data from placental tissues of pregnant women naturally infected with Zika virus during the third trimester were compared to those from women who tested negative for Zika infection. The findings were validated using both a cell culture model and an immunohistochemistry/morphological analysis of naturally infected placental tissues. Results: Transcriptome analysis revealed that Zika virus infection induces downregulation of insulin-like growth factor II (IGF2) gene, an essential factor for fetal development. The Caco-2 cell culture model that constitutively expresses IGF2 was used for the transcriptome validation. Asiatic and African Zika virus strains infection caused downregulated IGF2 gene expression in Caco-2 cells, whereas other flaviviruses, such as dengue serotype 1, West Nile and wild-type yellow fever viruses, had no effect on this gene expression. Immunohistochemical assays on decidual tissues corroborated our transcriptome analysis, showing that IGF2 is reduced in the decidua of Zika virus-infected women. Conclusions: Our results draw attention to IGF2 modulation in uterine tissues, and this finding is expected to support future studies on strategies to ameliorate the harmful effects of Zika virus infection during pregnancy.
dc.formatapplication/pdf
dc.languagepor
dc.publisherBritish Infection Society; Elsevier
dc.rightsopen access
dc.subjectZika Virus
dc.subjectTranscriptome
dc.subjectInsulin-Like Growth Factor II
dc.subjectDecidua
dc.subjectVirus Zika
dc.subjectTranscriptoma
dc.subjectFactor II del Crecimiento Similar a la Insulina
dc.subjectDecidua
dc.subjectFacteur de croissance IGF-II
dc.subjectCaduques
dc.subjectZika virus
dc.subjectPlacenta
dc.subjectTranscriptoma
dc.subjectFator de Crescimento Insulin-Like II
dc.subjectDecídua
dc.titleDownregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil
dc.typeArticle


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