Membrane-shed vesicles from the parasite Trichomonas vaginalis: characterization and their association with cell interaction

dc.creatorNievas, Yesica R.
dc.creatorCoceres, Veronica M.
dc.creatorMidlej, Victor
dc.creatorSouza, Wanderley de
dc.creatorBenchimol, Marlene
dc.creatorPereira-Neves, Antonio
dc.creatorVashisht, Ajay A.
dc.creatorWohlschlegel, James A.
dc.creatorJohnson, Patricia J.
dc.creatorMiguel, Natalia de
dc.date2019-05-22T13:09:23Z
dc.date2019-05-22T13:09:23Z
dc.date2018
dc.date.accessioned2023-09-26T23:21:10Z
dc.date.available2023-09-26T23:21:10Z
dc.identifierNIEVAS, Yesica R. et al. Membrane-Shed Vesicles from the Parasite Trichomonas Vaginalis: Characterization and Their Association with Cell Interaction. Cellular and Molecular Life Sciences, v. 75, n. 12, p. 2211–2226, 1 Jun. 2018.
dc.identifier1420-9071
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33171
dc.identifier10.1007/s00018-017-2726-3
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889635
dc.descriptionSubsídio da Agência Nacional de Promoção Científica e Tecnológica (ANPCyT), doação BID PICT 2013–1184 (NdM), uma subvenção colaborativa do Consejo Nacional de Investigações Científicas e Técnicas (CONICET) e da Fundação de Amparo à Pesquisa do O Estado do Rio de Janeiro (FAPERJ) (NdM e MB) e um Instituto Nacional de Subsídios de Saúde (NIH) AI103182 (PJJ). NdM e VMC são pesquisadores do Conselho Nacional de Pesquisa (CONICET) e da UNSAM. YRN é um PhD fellow do CONICET.
dc.descriptionTrichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Despite the serious consequences associated with trichomoniasis disease, little is known about parasite or host factors involved in attachment of the parasite-to-host epithelial cells. Here, we report the identification of microvesicle-like structures (MVs) released by T. vaginalis. MVs are considered universal transport vehicles for intercellular communication as they can incorporate peptides, proteins, lipids, miRNA, and mRNA, all of which can be transferred to target cells through receptor-ligand interactions, fusion with the cell membrane, and delivery of a functional cargo to the cytoplasm of the target cell. In the present study, we demonstrated that T. vaginalis release MVs from the plasma and the flagellar membranes of the parasite. We performed proteomic profiling of these structures demonstrating that they possess physical characteristics similar to mammalian extracellular vesicles and might be selectively charged with specific protein content. In addition, we demonstrated that viable T. vaginalis parasites release large vesicles (LVs), membrane structures larger than 1 µm that are able to interact with other parasites and with the host cell. Finally, we show that both populations of vesicles present on the surface of T vaginalis are induced in the presence of host cells, consistent with a role in modulating cell interactions.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectMicrovesicles
dc.subjectLarge vesicles
dc.subjectCellular communication
dc.subjectParasite
dc.subjectTrichomonas vaginalis
dc.subjectComunicação Celular
dc.subjectVesículas extracelulares / química
dc.subjectVesículas extracelulares / metabolismo
dc.subjectVesículas extracelulares / ultraestrutura
dc.subjectFêmea
dc.subjectCélulas HeLa
dc.subjectInterações Host-Parasite
dc.subjectHumanos
dc.subjectProteômica
dc.subjectProteínas de protozoários / análise
dc.subjectProteínas Protozoárias / metabolismo
dc.subjectTrichomonas Vaginite / metabolismo
dc.subjectTrichomonas Vaginite / parasitologia
dc.subjectTrichomonas vaginalis / química
dc.subjectTrichomonas vaginalis / citologia
dc.subjectTrichomonas vaginalis / fisiologia
dc.subjectTrichomonas vaginalis / ultraestrutura
dc.titleMembrane-shed vesicles from the parasite Trichomonas vaginalis: characterization and their association with cell interaction
dc.typeArticle


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